Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Catenin antibody [E247]

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Penguin Tissue sections (brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
Permeabilization No
Specification brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde

Other product details

Incubation time 2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS plus 1xcasein
Dilution 1/250

Secondary antibody

Name Non-Abcam antibody was used: Immpress universal
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

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Submitted May 03 2016