Western blot abreview for Anti-beta Catenin antibody [E247]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12 Cells)
Gel Running Conditions
Reduced Denaturing (4-15)
Loading amount
40 µg
Specification
C2C12 Cells
Blocking step
Sea Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Blocking Buffer + 0.2% Tween
Dilution
1/5000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye Goat Anti Rabbit 800 CW IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Infrared
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 86 kDa

Additional data

Additional Notes
Excellent antibody. It works perfectly for the WB application. I tested the following dilutions 1:2000, 1:5000, 1:8000. The results were comparable. Therefore, I would recommend to use 1:8000 to save on antibody. This is a general guideline only, concentration and optimization need to be performed based on the cells/tissue.
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Submitted Jun 06 2016

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