Non-Abcam antibody was used: IRDye Goat Anti Rabbit 800 CW IgG (H+L) Host species: Goat Clonality: Polyclonal Conjugation: IRDye® 800CW
4 minute(s) and 0 second(s)
Specific: 86 kDa
Excellent antibody. It works perfectly for the WB application. I tested the following dilutions 1:2000, 1:5000, 1:8000. The results were comparable. Therefore, I would recommend to use 1:8000 to save on antibody. This is a general guideline only, concentration and optimization need to be performed based on the cells/tissue.
Abcam user community
Submitted Jun 06 2016
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