Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Catenin antibody [E247]

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Mammary epithelial cells)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: 10mM citrate buffer
Permeabilization No
Specification Mammary epithelial cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Formaldehyde

Other product details

Incubation time 18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% non-immune goat serum
Dilution 1/800

Secondary antibody

Dilution 1/400
Name Non-Abcam antibody was used: Alexa Fluor 594 anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Abcam response Thank you for your valuable feedback about the use of this antibody in pig, a species that has not been tested before. We welcome any further feedback and AbReviews from other customers who have used this antibody in pig which will help us assess how well the antibody works in this species.

Mrs. Katie Elliott

Verified customer

Submitted Aug 15 2016