Western blot abreview for Anti-beta Catenin antibody [E247]

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human breast cancer cells)
Gel Running Conditions Reduced Denaturing (Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 50 µl)
Loading amount 80 µg
Specification human breast cancer cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time 18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution 1/5000

Secondary antibody

Dilution 1/2000
Name Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method HRP Vector VIP kit
Exposure 10 minute(s) and 0 second(s)
Bands Specific: 95 kDa
Positive control n/a
Username

Mrs. Katie Elliott

Verified customer

Submitted Aug 01 2016