Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human breast cancer cells)
Gel Running Conditions
Reduced Denaturing (Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 50 µl)
Loading amount
80 µg
Specification
human breast cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
HRP Vector VIP kit
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 95 kDa
Positive control
n/a
