Overview
- Product nameAnti-beta Catenin antibodySee all beta Catenin primary antibodies ...
- DescriptionRabbit polyclonal to beta Catenin
- Tested applicationsIHC-P, ICC/IF, IHC-FoFr, Sandwich ELISA, WB more details
- Species reactivityReacts with: Mouse, Rat, Human, Xenopus laevis, Squirrel
Predicted to work with: Chicken, Pig, Zebrafish
- Immunogen
Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human beta Catenin.
(Peptide available as ab16377.)
- Positive controlab16051 gave a positive result in the following whole cell lysates: Hela ab16051 gave a positive result in the following tissue lysates: Mouse Brain Mouse Testis Mouse Spinal Cord Mouse Ovary Rat Brain ab16051 gave a positive result in the following FFPE tissue; Human colon cancer.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
-
Research Areas
Applications
Our Abpromise guarantee covers the use of ab16051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. |
| IHC-FoFr | IHC-FoFr: 1/100. |
| Sandwich ELISA | sELISA: Use a concentration of 0.1 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.1 µg/ml with Mouse monoclonal [BDI080] to beta Catenin (ab19448) as Capture. |
| WB | WB: Use a concentration of 0.25 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).Can be blocked with beta Catenin peptide (ab16377). |
Target
- FunctionKey dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. - Tissue specificityExpressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
- Involvement in diseaseDefects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1. - Sequence similaritiesBelongs to the beta-catenin family.
Contains 12 ARM repeats. - Post-translational
modificationsPhosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. - Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
-
Database links
- Entrez Gene: 395964 Chicken
- Entrez Gene: 1499 Human
- Entrez Gene: 12387 Mouse
- Entrez Gene: 397657 Pig
- Entrez Gene: 84353 Rat
- Entrez Gene: 380441 Xenopus laevis
- Entrez Gene: 30265 Zebrafish
- Omim: 116806 Human
- SwissProt: P35222 Human
- SwissProt: Q02248 Mouse
- SwissProt: Q9WU82 Rat
- SwissProt: P26233 Xenopus laevis
- Unigene: 476018 Human
- Unigene: 291928 Mouse
- Unigene: 112601 Rat
see all
Target information above from: UniProt accession
P35222
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- b-catenin antibodyBeta catenin antibodyBeta-catenin antibody
- Cadherin associated protein antibodyCatenin (cadherin associated protein), beta 1, 88kDa antibodyCatenin beta 1 antibodyCatenin beta-1 antibodyCATNB antibodyCHBCAT antibodyCTNB1_HUMAN antibodyCTNNB antibodyCTNNB1 antibodyDKFZp686D02253 antibodyFLJ25606 antibodyFLJ37923 antibodyOTTHUMP00000162082 antibodyOTTHUMP00000165222 antibodyOTTHUMP00000165223 antibodyOTTHUMP00000209288 antibodyOTTHUMP00000209289 antibody
see all
Anti-beta Catenin antibody images
-
Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)ab16051 staining β-catenin in SW480 cells treated with XAV939 (ab120897), by ICC/IF. Increase of ß-catenin cytoplasmic expression and decrease in nuclear expression correlates with increased concentration of XAV939, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab120897 (XAV939) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab16051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
-
Sandwich ELISA - beta Catenin antibody (ab16051)Standard Curve for Beta-Catenin (Analyte: beta Catenin protein (Tagged) (ab63175)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [BDI080] to beta Catenin (ab19448) at 1ug/ml and Detector Antibody Rabbit polyclonal to beta Catenin (ab16051) at 0.1ug/ml. -
Western blotAll lanes : Anti-beta Catenin antibody (ab16051) at 0.25 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate withbeta Catenin peptide (ab16377) at 1 µg/ml A second band of 75 kDa was also detected in Hela whole cell lysates and A431 lysates. This smaller band was of equal intensity to the 94kDa band in the A431 lysates (data not shown).
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 94 kDa
Observed band size : 94 kDa
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - beta Catenin antibody (ab16051)ICC/IF image of ab16051 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16051X, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
-
Immunocytochemistry/ Immunofluorescence - beta Catenin antibody (ab16051)This image is courtesy of an Abreview submitted by Miss Helen Gillinghamab16051 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to incubation with the primary antibody (at 10µg/ml) for 1 hour at 27°C. A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary.
-
Immunohistochemistry (PFA perfusion fixed frozen sections) - beta Catenin antibody (ab16051)This image is a courtesy of Sophie Pezetab16051 staining beta Catenin in rat hypothalamus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat. The sample was incubated with primary antibody (1/100) for 18 hours at 200C in PBS + 0.3 % Triton X100. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/1000 dilution.
-
Western blot - Anti-beta Catenin antibody (ab16051)Anti-beta Catenin antibody (ab16051) at 0.25 µg/ml +beta Catenin protein (Tagged) (ab63175) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)IHC image of beta Catenin staining in Human colon cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16051, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References for Anti-beta Catenin antibody (ab16051)
This product has been referenced in:
- Yates LL et al. Scribble is required for normal epithelial cell-cell contacts and lumen morphogenesis in the mammalian lung. Dev Biol 373:267-80 (2013). Read more (PubMed: 23195221) »
- Neill T et al. Decorin antagonizes the angiogenic network: concurrent inhibition of Met, hypoxia inducible factor 1a, vascular endothelial growth factor A, and induction of thrombospondin-1 and TIMP3. J Biol Chem 287:5492-506 (2012). WB ; Human . Read more (PubMed: 22194599) »
