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Overview

  • Product nameAnti-beta Catenin antibodySee all beta Catenin primary antibodies ...
  • Description
    Rabbit polyclonal to beta Catenin
  • Tested applicationsELISA, IP, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (coupled to carrier protein) corresponding to amino acid residues in the N terminal region of human Beta Catenin.

  • Positive controlHct116 src transformed cells , human endothelial, A431 and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab23512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/2000.
IP IP: Use at an assay dependent dilution.
WB WB: 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 86 kDa).

Target

  • FunctionKey dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Tissue specificityExpressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • Involvement in diseaseDefects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similaritiesBelongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • Post-translational
    modifications    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.

    • Target information above from: UniProt accession P35222 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Beta catenin antibodyBeta-catenin antibodyCadherin associated protein antibody
        Catenin (cadherin associated protein), beta 1, 88kDa antibodyCatenin beta 1 antibodyCatenin beta-1 antibodyCATNB antibodyCHBCAT antibodyCTNB1_HUMAN antibodyCTNNB antibodyCTNNB1 antibodyDKFZp686D02253 antibodyFLJ25606 antibodyFLJ37923 antibodyOTTHUMP00000162082 antibodyOTTHUMP00000165222 antibodyOTTHUMP00000165223 antibodyOTTHUMP00000209288 antibodyOTTHUMP00000209289 antibody
      see all

    Anti-beta Catenin antibody images

    • Western blot - beta Catenin antibody (ab23512)
      Western blot - beta Catenin antibody (ab23512)
      Lanes 1 - 2 : Anti-beta Catenin antibody (ab23512) at 1/1000 dilution
      Lanes 3 - 4 : Anti-beta Catenin (phospho Y142) antibody (ab27798)

      Lane 1 : Hct116 src transformed cells (20 ug/lane) serum starved overnight
      Lane 2 : Hct116 src transformed cells (20 ug/lane) serum treated with pervanadate (1 mM) for 30 min.
      Lane 3 : Hct116 src transformed cells (20 ug/lane) serum starved overnight
      Lane 4 : Hct116 src transformed cells (20 ug/lane) serum treated with pervanadate (1 mM) for 30 min.


      Predicted band size : 86 kDa

    References for Anti-beta Catenin antibody (ab23512)

    ab23512 has not yet been referenced specifically in any publications.

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