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Overview

  • Product namebeta Catenin protein (Tagged)See all beta Catenin proteins and peptides ...
  • Protein descriptionRecombinant full length beta Catenin protein (Human), expressed by baculovirus in Sf9 insect cells, with N terminal GST tag, MW ~115kDa.
  • Expression hostBaculovirus infected sf9 cells

    • Properties

  • Purity> 90 % by SDS-PAGE
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab63175 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Ab63175 can be used as a WB positive control in conjunction with ab16051.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab16051.

    • Protein info

    • Alternative names
        Beta cateninBeta-cateninCadherin associated protein
        Catenin (cadherin associated protein), beta 1, 88kDaCatenin beta 1Catenin beta-1CATNBCHBCATCTNB1_HUMANCTNNBCTNNB1DKFZp686D02253FLJ25606FLJ37923OTTHUMP00000162082OTTHUMP00000165222OTTHUMP00000165223OTTHUMP00000209288OTTHUMP00000209289
      see all
  • FunctionKey dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Tissue specificityExpressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • Involvement in diseaseDefects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similaritiesBelongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • Post-translational
    modifications    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.

    • Target information above from: UniProt accession P35222 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    beta Catenin protein (Tagged) images

    • SDS-PAGE - beta Catenin protein (Tagged) (ab63175)
      SDS-PAGE - beta Catenin protein (Tagged) (ab63175)
      ab63175 on SDS-PAGE, MW ~115kDa.
    • Sandwich ELISA - beta Catenin protein (Tagged) (ab63175)
      Sandwich ELISA - beta Catenin protein (Tagged) (ab63175)
      Standard Curve for Beta-Catenin (Analyte: beta Catenin protein (Tagged) (ab63175)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [BDI080] to beta Catenin (ab19448) at 1ug/ml and Detector Antibody Rabbit polyclonal to beta Catenin (ab16051) at 0.1ug/ml.
    • Western blot - Anti-beta Catenin antibody (ab16051)
      Western blot - Anti-beta Catenin antibody (ab16051)
      Anti-beta Catenin antibody (ab16051) at 0.25 µg/ml + beta Catenin protein (Tagged) (ab63175) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

    References for beta Catenin protein (Tagged) (ab63175)

    ab63175 has not yet been referenced specifically in any publications.

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