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beta Defensin 1 protein (Active) (ab50048)

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Overview

  • Product namebeta Defensin 1 protein (Active)See all beta Defensin 1 proteins and peptides ...
  • Protein descriptionRecombinant Human beta-Defensin 1, a 3.9 kDa protein containing 36 amino acid residues.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesEndotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Biological activityBiological Activity : Determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 100.0-1000.0 ng/ml.
  • FormLyophilised
  • Storage instructionsThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Sequence notesDHYNCVSSGG QCLYSACPIF TKIQGTCYRG KAKCCK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab50048 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab14425.

    • Protein info

    • Alternative names
        BD 1BD-1BD1
        beta 1Beta defensin 1Beta-defensin 1DEFB 1DEFB1DEFB1_HUMANDEFB101DefensinDefensin beta 1Defensin beta 1 preproproteinHBD 1hBD-1HBD1MGC51822
      see all
  • FunctionHas bactericidal activity.
  • Tissue specificityPlasma.
  • Sequence similaritiesBelongs to the beta-defensin family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P60022 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for beta Defensin 1 protein (Active) (ab50048)

    ab50048 has not yet been referenced specifically in any publications.

    Publishing research using ab50048 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab50048 Submit a Question for ab50048

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    Are these proteins tagged? Do they contain any disulfide bonds / protein folding or are they linear?

    Thank you for contacting us. Neither of these proteins are tagged. Both ab50048 and ab50058 both contain a six-cysteine motif that forms three intra-molecular disulfide bonds. I hope this helps, please let me know if you need any additional information or...

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    Customer has performed the Dot-Blotprotocolas suggested;please findthe results attached.

    Regarding the storage:
    - antibodies were stored at -20°C.
    - recombinant proteins were reconstituted with 100 ul PBS + 15% glycerol, aliq...

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    Thank you for sending along the dot blots and additional information. We really appreciate the extra troubleshootingeffort.

    Here are my thoughts:

    1. In general it looks like the antibodies are able to detect Defensin in the chorioamnio...

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    For the technical support,   Please, customer is having problems using human beta defensin antibodies and their respective recombinant proteins in WB applications.   Find bellow the technical questionnaire filled out by the customer.   As the beta def...

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    Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is are having problems with 8 of our products.  Special thanks for collecting the troubled ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"