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Anti-beta Galactosidase antibody [BG-02] (ab1047)

Abreviews (2)Q&A (17)Specific References (6)

Overview

  • Product nameAnti-beta Galactosidase antibody [BG-02]See all beta Galactosidase primary antibodies ...
  • Description
    Mouse monoclonal [BG-02] to beta Galactosidase
  • Tested applicationsWB, ICC, ICC/IF more details
  • Immunogen

    Full length native protein (purified) (E. coli).

  • EpitopeRecognizes a non-catalytic centre epitope of Beta-galactosidase from E. coli.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by precipitation methods.
  • Clonality Monoclonal
  • Clone numberBG-02
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab1047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent concentration.
ICC ICC: Use at an assay dependent dilution.
ICC/IF ICC/IF: Use at an assay dependent concentration. Used at a dilution of 1/50 for 18 hrs on mouse mammary cells (see Abreview for further information).

Target

  • RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localizationCytoplasmic
  • Alternative names
      Beta D galactosidase antibodyBeta gal antibodyBeta galactosidase antibody
      EC 3.2.1.23 antibodyECK0341 antibodyJW0335 antibodylactase antibodylacZ antibody
    see all

References for Anti-beta Galactosidase antibody [BG-02] (ab1047)

This product has been referenced in:

See all 6 Publications for this product

Publishing research using ab1047 ? Please let us know so that we can cite the reference in this datasheet

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Displaying 1 - 10 of 19 results for Abreviews and Q&A

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What is the size of the band in WB? FAQ mentions publication but customer has no access



We also do not have electronic access to the publication

Folia Biol (Praha). 1992;38(6):350-7.

A novel panel of monoclonal antibodies against beta-galactosidase of Escherichia coli and its versatility for detection of recombi...

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Our customer wonders if ab1047 will recognize both over expressed beta Galactosidase and also beta Galactosidase tagged protein.

The Mouse monoclonal [BG-02] to beta Galactosidase, ab1047, should be able to detect the beta Galactosidase with the same sequence as that expressed in E. coli. This should work whether the protein is overexpressed or not. It should also work with beta Ga...

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Thanks for your reply. Please send us the chick antibody free of charge as approved by XXXXXX. Regards,  

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cause...

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Thanks very much for reviewing this case and offering us the chance to try another b-gal antibody. We would be grateful to receive a vial of AB9361, raised in chick. We’ll just have to find a good secondary antibody. With best regards,  

If Chicken secondary is a problem then I would suggest choosing other beta galactosidase antibodies from the catalogue, with other host e.g. rabbit as ab4761. I hope this will help.   

Western blot abreview for Anti-beta Galactosidase antibody [BG-02]

 Poor
Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (whole brain)
Loading amount 50 µg
Specification whole brain
Treatment Samples from wild-type (control) mice and gene-trap mice expressing b-geo fusion proteins
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Verified customer

Submitted Jul 31 2007

This anitbody data sheet has an image that shows positive ICC results in mammary cells, but there is no species reactivity listed. Would this antibody be suitable for western blot with mouse mammalian cells?

Thank you for your enquiry. This antibody shows no cross-reactivity with other E. coli proteins and can be used for identification and characterization of beta-galactosidase fusion proteins expressed in lambda expression vectors. It was successfully u...

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I am using a construct that codes for the alpha fragment of beta galactosidase. Will this antibody recognize this?

Thank you for your enquiry. I am sorry I can not answer the question; however, I did find the following information in a product specific publication for this clone by Slavickova et al., PubMed ID 1284241. In this publication I have found these dat...

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Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Galactosidase antibody [BG-02]

 Excellent
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mammary)
Specification Mammary
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted Apr 05 2006

I have a customer who wants me to confirm that this antibody has successfully been used in immunofluorescence on guinea pig tissue. I know this is mentioned in the datasheet, but not directly and she wants me to confirm this.

Thank you for your enquiry. To our knowledge, our beta Galactosidase antibody [BG-02]has yet to be tested by immunohistochemistry or on guinea pig tissue. All tested applications are specified on Abcam product datasheets. Should you require furthe...

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Customer has reported that this antibody did not work for her in IF. She used mouse neural stem cells and used ab1047 side by side with another company's beta gal antibody. With that ab, she saw nice specific staining but with ab1047 just saw background s...

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Thank you for your phone call and I'm sorry to hear that you have experienced difficulty with ab1047. As we discussed over the phone, I can have a refund issued to you for your purchase. I have instructed our accounting department to issue the refun...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"