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Products:Tags & Cell Markers >> Fusion / Marker Proteins >> Beta Galactosidase
Overview
Product name
Anti-beta Galactosidase antibody (FITC)
See all beta Galactosidase products (18) ...
Description
Rabbit polyclonal to beta Galactosidase (FITC)
Conjugation
FITC
Conjugation notes
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Rabbit IgG
Tested applications
IHC-Fr, IHC (Methanol fixed), ICC/IFmore details
Immunogen
Full length protein (E. coli).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02M Potassium Phosphate, 0.15M NaCl (pH 7.2) with 1% BSA, 50% glycerol and 0.01% Sodium Azide
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Fusion / Marker Proteins >> Beta Galactosidase
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Immunocytochemistry - beta Galactosidase antibody (ab6641)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab6641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (Methanol fixed)
ICC/IF
Application notes
ICC/IF: 1/500 - 1/2500.
IHC-Fr: 1/200.
Immunohistochemistry (Methanol fixed cells): 1/500 (from PubMed:16586440).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Cellular localization
Cytoplasmic
Alternative names
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
Anti-beta Galactosidase antibody (FITC) images:
Immunocytochemistry - beta Galactosidase antibody (ab6641)
Ab6641 positively staining beta galactosidase formaldehyde fixed mouse heart frozen sections (1/200).
This image is courtesy of an Abreview submitted on 14th October 2005. We do not have any further information relating to this image.
Protocols
References for Anti-beta Galactosidase antibody (FITC) (ab6641)
This product has been referenced in:
- Luheshi NMet al. The dynamics and mechanisms of interleukin-1alpha and beta nuclear import. Traffic 10:16-25 (2009). ICC/IF.Read more (PubMed: 18939951) »
- Deal KKet al. Distant regulatory elements in a Sox10-beta GEO BAC transgene are required for expression of Sox10 in the enteric nervous system and other neural crest-derived tissues. Dev Dyn 235:1413-32 (2006).Read more (PubMed: 16586440) »
See all 4 publications for this product
Publishing research using ab6641? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"