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Overview

  • Product nameAnti-beta Galactosidase antibodySee all beta Galactosidase primary antibodies ...
  • Description
    Goat polyclonal to beta Galactosidase
  • SpecificityDetects recombinant beta galactosidase enzyme by Western blotting.
  • Tested applicationsWB, IHC-Fr more details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant full length protein of Escherichia coli beta Galactosidase.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab12081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa.
IHC-Fr IHC-Fr: Use at an assay dependent dilution.

Target

  • RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localizationCytoplasmic
  • Database links
    • Alternative names
        Beta D galactosidase antibodyBeta gal antibodyBeta galactosidase antibody
        EC 3.2.1.23 antibodyECK0341 antibodyJW0335 antibodylactase antibodylacZ antibody
      see all

    Anti-beta Galactosidase antibody images

    • Western blot - Anti-beta Galactosidase antibody (ab12081)
      Western blot - Anti-beta Galactosidase antibody (ab12081)
      Anti-beta Galactosidase antibody (ab12081) at 1.5 µg/ml + Beta Galactosidase Recombinant at 0.1 µg

      Secondary
      Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 116 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 90 seconds

    References for Anti-beta Galactosidase antibody (ab12081)

    This product has been referenced in:

    See all 3 Publications for this product

    Publishing research using ab12081 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Western blot abreview for Anti-beta Galactosidase antibody

     Excellent
    Abreviews
    Application Western blot
    Sample Escherichia coli Cell lysate - whole cell (bact beta-gal protein expressed in rat epithelial)
    Loading amount 20 µg
    Specification bact beta-gal protein expressed in rat epithelial
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
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    Verified customer

    Submitted Aug 13 2012

    Inquiry: Hello. I just received a shipment of ab12081 Goat anti LacZ lot. As I was aliquoting the antibody for long term storage at -20 I only was able to retrieve 70uL of antibody but there should be 100ul in the vial. The vial was centrifuged before ope...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not with the full amount as stated on the datasheet and for the inconvenience th...

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    My Fluorescent signal is weak using xylene based mounting media.

    Thank you for your phone call.

    As we discussed, I recommend using aqueous mounting media for fluorescent detection, such as ab104139. These media are often specially formulated to preserve the fluorescence and simultaneously counterstain with DA...

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    Thanks for your help on this matter. Much appreciated.  I have attached an email showing the purchase of this anti lacZ antibody from March 24 2011... just short of 6 months.  The current lot # we are using is 134764. Would you happen to kno...

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    Thank you for your email. Unfortunately we only have the same lot in stock, would you like a replacement from the same lot or an alternative product? We will be able to organise the replacement with Cedarlane. Looking forward to your reply...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"