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Products:Tags & Cell Markers >> Fusion / Marker Proteins >> Beta Galactosidase
Overview
Product name
Anti-beta Galactosidase antibody
See all beta Galactosidase products (18) ...
Description
Rabbit polyclonal to beta Galactosidase
Specificity
Detects recombinant beta Galactosidase in Western blotting. When used in Western blotting on 20ug Hela cell lysate dosed with 0.1ug of beta Galactosidase, detects a single strong band corresponding to beta Galactosidase.
Tested applications
IHC-P, WBmore details
Immunogen
Full length native protein (purified) (E. coli).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Fusion / Marker Proteins >> Beta Galactosidase
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Western blot - beta Galactosidase antibody (ab4761)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab4761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
- 1 Image
WB
Application notes
WB: Use at an assay dependent dilution. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).
IHC-P: Use at an assay dependent dilution from (PubMed:17268532).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Cellular localization
Cytoplasmic
Alternative names
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
Anti-beta Galactosidase antibody images:
Western blot - beta Galactosidase antibody (ab4761)
All lanes : Anti-beta Galactosidase antibody (ab4761) at 1/1000 dilution
Lane 1 : Beta Gal recombinant protein at 0.1 µg
Lane 2 : 20ug HeLa WCL + 0.1ug Beta Gal recombinant protein at 20 µg
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 116 kDa
Observed band size : 116 kDa
References for Anti-beta Galactosidase antibody (ab4761)
This product has been referenced in:
- Debrand Eet al. Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms. FEBS J 276:1610-28 (2009). WB.Read more (PubMed: 19220457) »
- Hachiya Aet al. Gene transfer in human skin with different pseudotyped HIV-based vectors. Gene Ther 14:648-56 (2007).Read more (PubMed: 17268532) »
See all 3 publications for this product
Publishing research using ab4761? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"