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Immunocytochemistry/ Immunofluorescence - beta Galactosidase antibody (ab616)
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Product Name
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beta Galactosidase antibody
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See all beta Galactosidase antibodies (15)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to beta Galactosidase
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Immunogen
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Recombinant full length protein (E. coli).
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Reacts with
(species key)
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E coli
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Specificity
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This polycolonal antibody reacts with beta-galactosidase from E.coli showing a 464 kDa band (tetramer) under non-reducing conditions of SDS-PAGE and 116 kDa band under reducing conditions. This antibody does not cross-react with mouse beta-galactosidase.
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Tested applications
(see key)
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ELISA, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000 - 1/25000 (sandwich ELISA tested against 1ug E.coli beta galactosidase.
IP: 1/500 - 1/5000.
WB: 1/5000 - 1/25000. Detects a band of approximately 464 kDa under non-reducing conditions (tetramer) and of approximately 116 kDa band under reducing conditions.
IHC-FoFr: Use at an assay dependent dilution (PMID 18403128).
This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results.
If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.
We would recommend ab4761 or ab12081 as alternatives.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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1.0µg of E.coli in a standard sandwich ELISA. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified E.coli beta-galactosidase.
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Cellular localization
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Cytoplasmic
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Research areas
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Tags & Cell Markers >> Fusion / Marker Proteins >> Beta Galactosidase
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Relevance
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Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 945006 (Escherichia coli )
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| SwissProt | | P00722 (Escherichia coli )
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 1% BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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IgG fraction purified from monospecific antiserum by a multi-step process of delipidation, salt fractionation and ion exchange chromatography and dialysis.
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Form
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Liquid
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Concentration
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10.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this beta Galactosidase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for beta Galactosidase antibody images, references, products related to ab616 and other tools.
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beta Galactosidase antibody images:
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 Immunocytochemistry/ Immunofluorescence - beta Galactosidase antibody (ab616)
ab616 at 1/400 staining mouse Rosa-Mesenchymal stem cells by ICC/IF. Mesenchymal stem cells (MSC) were collected from the bone marrow of Rosa mice for b-galactosidase staining. Rosa mice are a line of transgenic mice expressing the LacZ gene controlled by a promoter, which directs expression to essentially all tissues (Rosa 26, Jackson Laboratories). The cells were 2% paraformaldehyde ffixed for 15 minutes and then permealized with Triton-X100 (0.1%) for 10 minutes and then incubated with ab616 overnight at 4°C. The image demonstrates b-galactosidase expressing MSC (green-cytoplasmic localization) and DAPI (blue-nuclear counter stain). This image is courtesy of an Abreview submitted by Dr Mal Niladri
See Abreview
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References for beta Galactosidase antibody (ab616)
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This product has been used in: (two most recent references)
Jacques TS et al. Combinations of genetic mutations in the adult neural stem cell compartment determine brain tumour phenotypes. EMBO J 29:222-35 (2010). IHC-P. PubMed: 19927122
Fouladkou F et al. The ubiquitin ligase Nedd4-1 is required for heart development and is a suppressor of thrombospondin-1. J Biol Chem 285:6770-80 (2010). PubMed: 20026598
See all 50 publication references for this product.
If you publish research using ab616 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to beta Galactosidase
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beta Galactosidase antibody - more information
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