Specific protocols
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To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you get on. |
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General protocols
Useful resources:Western blotting (WB) protocols:Immunohistochemistry (IHC) / Immunocytochemistry (ICC) protocols:Chromatin Immunoprecipitation (ChIP) protocols:Dot blot protocols:ELISA protocols:ELISPOT protocols: Flow cytometry / FACS protocols:Immunoprecipitation (IP) protocols: |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - beta Galactosidase antibody (ab9361)
ab9361 staining beta Galactosidase in fruit fly central nervous system glia cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton. Samples were then incubated with primary antibody at 1/2000 for 12 hours at 4ºC. The secondary antibody used was a donkey anti-chicken monoclonal conjugated to DyLight® 649 (pink) used at a 1/400 dilution. Nuclei stained with a pan nuclear marker (green).
Image courtesy of Dr Sean Speese by Abreview.
Immunohistochemistry (PFA perfusion fixed frozen sections) - beta Galactosidase antibody (ab9361)
ab9361 at 1/500 dilution staining mouse adult brain cells (R26R mice crossed with cre expressing line) by Immunocytochemistry. The cells were 4% PFA perfused 4H post-fix. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using a Alexa Fluor ® 488 conjugated goat-anti chicken antibody. The image shows both nuclear and punctate staining (both matched x-gal staining pattern).
This image is courtesy of an Abreview submitted by Ben Deverman.
Immunohistochemistry (Frozen sections) - beta Galactosidase antibody (ab9361)
ab9361 at 1/1000 staining frozen mouse pancreas sections by IHC-Fr. The mice were transgenic knock out or heterozygous (in this case LRH +/-)with Lacz reporter replacing a section of the coding sequence for gene. Embryos collected at day E14.5. The tissue was formaldehyde fixed and blocked with a maleate buffer blocking solution prior to incubation with the antibody for 16 hours. A Cy3 ® conjugated goat anti-chicken antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Asha Seth
Immunocytochemistry/ Immunofluorescence - beta Galactosidase antibody (ab9361)
ab9361 at 1/250 staining human HEK293T cells by ICC/IF. The cell line was transfected with a b-gal expressing plasmid, and x-gal staining was performed on adjacent wells. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours. A Texas Red conjugated donkey anti-chicken antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Deon Wolpowitz
Immunohistochemistry (Frozen sections) - beta Galactosidase antibody (ab9361)
ab9361 staining Beta Galactosidase in mouse retinal tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.2% triton-X and blocked with 5% serum for 1 hour at 23ºC. The sample was incubated with primary antibody (1/1500 in PBS, 2% serum, 0.2% Triton-X) at 4ºC for 16 hours. An Alexa Fluor®488-conjugated Goat monoclonal to chicken IgY (1/200) was used as secondary antibody.
This image is courtesy of an Abreview submitted by Dr Steven Hughes
beta Galactosidase antibody for Immunohistochemistry (PFA perfusion fixed frozen sections) in Mouse (9361)
beta Galactosidase antibody for IHC-Fr in Mouse (9361)
beta Galactosidase antibody for IHC-Fr in Mouse (9361)
beta Galactosidase antibody for Immunohistochemistry (Frozen sections) in Mouse (9361)
beta Galactosidase antibody for IHC-Fr in Mouse (9361)
beta Galactosidase antibody for ICC/IF in Mouse (9361)
beta Galactosidase antibody for ICC in Human (9361)
beta Galactosidase antibody for IHC-Fr in Mouse (9361)
beta Galactosidase antibody for IHC-FrFl in Mouse (9361)
beta Galactosidase antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (9361)
beta Galactosidase antibody for IHC-Fr in Mouse (9361)
beta Galactosidase antibody for IHC-P in Mouse (9361)
beta Galactosidase antibody for Immunocytochemistry/ Immunofluorescence in Fruit fly (Drosophila melanogaster) (9361)
Anti-beta Galactosidase antibody for Immunocytochemistry/ Immunofluorescence in Fruit fly (Drosophila melanogaster) (9361)
beta Galactosidase antibody for IHC-Fr in Mouse (9361)
Anti-beta Galactosidase antibody for Immunocytochemistry/ Immunofluorescence in Mouse (9361)
Anti-beta Galactosidase antibody for Immunocytochemistry/ Immunofluorescence in Rat (9361)
beta Galactosidase antibody for IHC-Fr in Mouse (9361)
Anti-beta Galactosidase antibody for Immunocytochemistry/ Immunofluorescence in Mouse (9361)
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