Anti-beta Galactosidase antibody (ab9361)

  Immunocytochemistry/ Immunofluorescence - beta Galactosidase antibody (ab9361)

ab9361 staining beta Galactosidase in fruit fly central nervous system glia cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton. Samples were then incubated with primary antibody at 1/2000 for 12 hours at 4°C. The secondary antibody used was a donkey anti-chicken monoclonal conjugated to DyLight® 649 (pink) used at a 1/400 dilution. Nuclei stained with a pan nuclear marker (green).

Image courtesy of Dr Sean Speese by Abreview.

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  Immunohistochemistry (PFA perfusion fixed frozen sections) - beta Galactosidase antibody (ab9361)

ab9361 at 1/500 dilution staining mouse adult brain cells (R26R mice crossed with cre expressing line)  by Immunocytochemistry. The cells were 4% PFA perfused 4H post-fix. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using a Alexa Fluor ® 488 conjugated goat-anti chicken antibody. The image shows both nuclear and punctate staining (both matched x-gal staining pattern).

This image is courtesy of an Abreview submitted by Ben Deverman.

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  Immunohistochemistry (Frozen sections) - beta Galactosidase antibody (ab9361)

ab9361 at 1/1000 staining frozen mouse pancreas sections by IHC-Fr. The mice were transgenic knock out or heterozygous (in this case LRH +/-)with Lacz reporter replacing a section of the coding sequence for gene. Embryos collected at day E14.5. The tissue was formaldehyde fixed and blocked with a maleate buffer blocking solution prior to incubation with the antibody for 16 hours. A Cy3 ® conjugated goat anti-chicken antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Asha Seth

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  Immunocytochemistry/ Immunofluorescence - beta Galactosidase antibody (ab9361)

ab9361 at 1/250 staining human HEK293T cells by ICC/IF. The cell line was transfected with a b-gal expressing plasmid, and x-gal staining was performed on adjacent wells. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours. A Texas Red conjugated donkey anti-chicken antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Deon Wolpowitz

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  Immunohistochemistry (Frozen sections) - beta Galactosidase antibody (ab9361)

ab9361 staining Beta Galactosidase in mouse retinal tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.2% triton-X and blocked with 5% serum for 1 hour at 23°C. The sample was incubated with primary antibody (1/1500 in PBS, 2% serum, 0.2% Triton-X) at 4°C for 16 hours. An Alexa Fluor^®488-conjugated Goat monoclonal to chicken IgY (1/200) was used as secondary antibody.

This image is courtesy of an Abreview submitted by Dr Steven Hughes

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