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Full length native protein (purified) (E. coli).
Our Abpromise guarantee covers the use of ab9361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-FrFl||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IHC-FoFr||1/1000 - 1/2000.|
|Flow Cyt||Use at an assay dependent concentration. Ab37382-Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 116 kDa.|
|IHC-P||1/200 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab9361 at 1/500 dilution staining mouse adult brain cells (R26R mice crossed with cre expressing line) by Immunocytochemistry. The cells were 4% PFA perfused 4H post-fix. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using a Alexa Fluor ® 488 conjugated goat-anti chicken antibody. The image shows both nuclear and punctate staining (both matched x-gal staining pattern).
This image is courtesy of an Abreview submitted by Ben Deverman.
ab9361 at 1/1000 staining frozen mouse pancreas sections by IHC-Fr. The mice were transgenic knock out or heterozygous (in this case LRH +/-)with Lacz reporter replacing a section of the coding sequence for gene. Embryos collected at day E14.5. The tissue was formaldehyde fixed and blocked with a maleate buffer blocking solution prior to incubation with the antibody for 16 hours. A Cy3 ® conjugated goat anti-chicken antibody was used as the secondary.
ab9361 staining beta Galactosidase in mouse e13 stomach and liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Davidson's fixative, permeabilized with 0.5% Triton-X 100 and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in TBST + 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-chicken IgY polyclonal (1/500) was used as the secondary antibody.
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