Overview

  • Product nameAnti-beta Galactosidase antibody
    See all beta Galactosidase primary antibodies
  • Description
    Chicken polyclonal to beta Galactosidase
  • Tested applicationsELISA, IHC-FrFl, ICC, IHC-FoFr, Flow Cyt, ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Full length native protein (purified) (E. coli).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.01M PBS pH7.4, 15mM Na azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were solid phase absorbed then immunoaffinity purified using purified beta-galactosidase immobilized on a solid phase.
  • Clonality Polyclonal
  • IsotypeIgY
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab9361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-FoFr 1/1000 - 1/2000.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 116 kDa.
IHC-P 1/200 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localizationCytoplasmic
  • Database links
    • Alternative names
      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • EC 3.2.1.23 antibody
      • ECK0341 antibody
      • JW0335 antibody
      • lactase antibody
      • lacZ antibody
      see all

    Anti-beta Galactosidase antibody images

    • ab9361 staining beta Galactosidase in fruit fly central nervous system glia cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton. Samples were then incubated with primary antibody at 1/2000 for 12 hours at 4°C. The secondary antibody used was a donkey anti-chicken monoclonal conjugated to DyLight® 649 (pink) used at a 1/400 dilution. Nuclei stained with a pan nuclear marker (green).

      See Abreview

    • ab9361 at 1/500 dilution staining mouse adult brain cells (R26R mice crossed with cre expressing line)  by Immunocytochemistry. The cells were 4% PFA perfused 4H post-fix. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using a Alexa Fluor ® 488 conjugated goat-anti chicken antibody. The image shows both nuclear and punctate staining (both matched x-gal staining pattern).

      This image is courtesy of an Abreview submitted by Ben Deverman.

      See Abreview

    • ab9361 at 1/1000 staining frozen mouse pancreas sections by IHC-Fr. The mice were transgenic knock out or heterozygous (in this case LRH +/-)with Lacz reporter replacing a section of the coding sequence for gene. Embryos collected at day E14.5. The tissue was formaldehyde fixed and blocked with a maleate buffer blocking solution prior to incubation with the antibody for 16 hours. A Cy3 ® conjugated goat anti-chicken antibody was used as the secondary.

      See Abreview

    • ab9361 at 1/250 staining human HEK293T cells by ICC/IF. The cell line was transfected with a b-gal expressing plasmid, and x-gal staining was performed on adjacent wells. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours. A Texas Red conjugated donkey anti-chicken antibody was used as the secondary.

      See Abreview

    • ab9361 staining Beta Galactosidase in mouse retinal tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.2% triton-X and blocked with 5% serum for 1 hour at 23°C. The sample was incubated with primary antibody (1/1500 in PBS, 2% serum, 0.2% Triton-X) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat monoclonal to chicken IgY (1/200) was used as secondary antibody.

      See Abreview

    • ab9361 staining beta Galactosidase in mouse e13 stomach and liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Davidson's fixative, permeabilized with 0.5% Triton-X 100 and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in TBST + 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-chicken IgY polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    References for Anti-beta Galactosidase antibody (ab9361)

    This product has been referenced in:
    • Di Giovannantonio LG  et al. Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity. Development 141:377-88 (2014). Read more (PubMed: 24335253) »
    • Cox BC  et al. Spontaneous hair cell regeneration in the neonatal mouse cochlea in vivo. Development 141:816-29 (2014). Read more (PubMed: 24496619) »

    See all 79 Publications for this product

    Product Wall

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (cultured acute spinal cord slice from Roza26-LacZ)
    Specification cultured acute spinal cord slice from Roza26-LacZ
    Permeabilization Yes - Triton X-100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 19 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric
    Sample Mouse Tissue sections (e13 stomach and liver)
    Specification e13 stomach and liver
    Permeabilization Yes - 0.5 % Triton-X 100
    Fixative Davidson's fixative
    Username

    Dr. Laurel Baglia

    Verified customer

    Submitted Mar 24 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (Mouse, whole brain sections, striatum)
    Specification Mouse, whole brain sections, striatum
    Permeabilization Yes - Tween-20
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 08 2013


    ab9361 was raised against E. coli beta galactosidase, which only has 11% homology with mouse beta galactosidase. In general, we like to see greater than 85% homology before we expect an antibody to cross react with an untested species. Thus, ab936...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the...

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    I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
    In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. O...

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    Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie besprochen, habe ich eine kostenlose Ersatzlieferung des ab97135 für Sie in Auftrag gegeben. Sie hat die Referenznummer 1212...

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