Overview

  • Product nameAnti-beta Galactosidase antibody
    See all beta Galactosidase primary antibodies
  • Description
    Chicken polyclonal to beta Galactosidase
  • Tested applicationsELISA, IHC-FrFl, ICC, IHC-FoFr, Flow Cyt, ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Full length native protein (purified) (E. coli).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer0.01M PBS pH7.4, 15mM Na azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were solid phase absorbed then immunoaffinity purified using purified beta-galactosidase immobilized on a solid phase.
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-FoFr 1/1000 - 1/2000.
Flow Cyt Use at an assay dependent concentration. ab37382-Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 116 kDa.
IHC-P 1/200 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localizationCytoplasmic
  • Database links
    • Alternative names
      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    Anti-beta Galactosidase antibody images

    • P0-adult mice were euthanized and perfused with 4% paraformaldehyde in PBS (PF). Their spinal cords were then post-fixed for 30–60 mins in 4% PF at 4°C (P0) or at room temperature (adult). Spinal cords were rinsed and cryoprotected in 20% sucrose in PBS (4°C) prior to embedding in OCT (Tissue-Tek). Immunostaining of frozen spinal sections was performed by incubating 20 µm thick sections with primary antibodies, which were then detected using species-specific secondary antibodies conjugated with Cy2, Cy3 and Cy5 or FITC. ab9361 was used at 1:1000.

    • ab9361 staining beta Galactosidase in mouse e13 stomach and liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Davidson's fixative, permeabilized with 0.5% Triton-X 100 and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in TBST + 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-chicken IgY polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    • ab9361 staining beta Galactosidase in fruit fly central nervous system glia cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton. Samples were then incubated with primary antibody at 1/2000 for 12 hours at 4°C. The secondary antibody used was a donkey anti-chicken monoclonal conjugated to DyLight® 649 (pink) used at a 1/400 dilution. Nuclei stained with a pan nuclear marker (green).

      See Abreview

    • Ab9361 staining Beta galactosidase in Mouse thyroid tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed 10% buffered formalin, cut into 3-4 micron slices, blocked with 10% normal goat serum and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with the following primary antibodies; anti-BrdU, anti-Sca1 (ab109211, 1∶600), anti-NKX2-1 and anti-β-gal (ab9361, 1∶1000). Sections were incubated with the first primary antibody (anti-Sca1) for 1 hour at room temperature. After washing with PBS, sections were incubated with the first secondary antibody (Alexa Fluor 555 goat anti-rabbit IgG) and washed with PBS. Sections were then incubated with normal serum (5% rabbit serum) from the same host species as the first primary antibodies for 1 hour at room temperature and washed with PBS. Sections were further incubated with an excess of unconjugated Fab antibody derived from the same host species as the primary antibody for 1 hour at room temperature and washed with PBS. The sections were finally incubated with the mixed second primary antibodies (anti-BrdU, anti-β-gal, anti-NKX2-1) overnight at 4°C, washed with PBS, and were incubated with the second secondary antibody (Alexa Fluor 488 goat anti-rat IgG, Dylight 650 goat anti-chicken IgY, Dylight 594 goat anti-rabbit IgG) for 1 hour at room temperature and washed with PBS. DAPI dye was used to stain the nuclei of cells.

    • ab9361 staining Beta Galactosidase in mouse retinal tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.2% triton-X and blocked with 5% serum for 1 hour at 23°C. The sample was incubated with primary antibody (1/1500 in PBS, 2% serum, 0.2% Triton-X) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat monoclonal to chicken IgY (1/200) was used as secondary antibody.

      See Abreview

    • ab9361 at 1/250 staining human HEK293T cells by ICC/IF. The cell line was transfected with a b-gal expressing plasmid, and x-gal staining was performed on adjacent wells. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours. A Texas Red conjugated donkey anti-chicken antibody was used as the secondary.

      See Abreview

    References for Anti-beta Galactosidase antibody (ab9361)

    This product has been referenced in:

    See all 108 Publications for this product

    Product Wall

    Application Immunohistochemistry - Free Floating
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Username

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    Verified customer

    Submitted Jan 12 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (E12.5 Embryo)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EGTA, pH=9, pressure cooker
    Permeabilization Yes - Saponi
    Specification E12.5 Embryo
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

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    Submitted Feb 16 2016

    Application Immunohistochemistry - Free Floating
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Username

    Abcam user community

    Verified customer

    Submitted Jan 14 2016

    Application Immunocytochemistry/ Immunofluorescence
    Sample Fruit fly (Drosophila melanogaster) Cell (Larval brain)
    Permeabilization Yes - TBS-T
    Specification Larval brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative Formaldehyde
    Username

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    Submitted Aug 20 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Intestine)
    Permeabilization Yes - .01 SDS
    Specification Intestine
    Blocking step Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative Paraformaldehyde
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    Submitted Jul 22 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Main Olfactory Epithelium (MOE))
    Permeabilization Yes - TritonX 0.01%
    Specification Main Olfactory Epithelium (MOE)
    Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Jennifer Cisson

    Verified customer

    Submitted May 11 2015

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (cultured acute spinal cord slice from Roza26-LacZ)
    Specification cultured acute spinal cord slice from Roza26-LacZ
    Permeabilization Yes - Triton X-100
    Fixative Paraformaldehyde
    Username

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    Submitted Jun 19 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric
    Sample Mouse Tissue sections (e13 stomach and liver)
    Specification e13 stomach and liver
    Permeabilization Yes - 0.5 % Triton-X 100
    Fixative Davidson's fixative
    Username

    Dr. Laurel Baglia

    Verified customer

    Submitted Mar 24 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (Mouse, whole brain sections, striatum)
    Specification Mouse, whole brain sections, striatum
    Permeabilization Yes - Tween-20
    Fixative Paraformaldehyde
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    Submitted Jul 08 2013


    ab9361 was raised against E. coli beta galactosidase, which only has 11% homology with mouse beta galactosidase. In general, we like to see greater than 85% homology before we expect an antibody to cross react with an untested species. Thus, ab936...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"