Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end (ab50148)
Key features and details
- Rabbit polyclonal to beta Glucuronidase (GUS) - Aminoterminal end
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end -
Description
Rabbit polyclonal to beta Glucuronidase (GUS) - Aminoterminal end -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Synthetic peptide corresponding to Escherichia coli Beta Glucuronidase (GUS) aa 1-16 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:MLRPVETPTREIKKLD
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa. Staining of the beta Glucuronidase band in immunoblotting is specifically inhibited by the immunizing peptide.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa. Staining of the beta Glucuronidase band in immunoblotting is specifically inhibited by the immunizing peptide. |
Target
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Relevance
Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3992859 Escherichia coli
- Entrez Gene: 946149 Escherichia coli
- SwissProt: P05804 Escherichia coli
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Alternative names
- Beta D glucuronidase antibody
- Beta glucuronidase antibody
- gurA antibody
- UidA antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab50148 has been referenced in 7 publications.
- Tu B et al. Loss of Gn1a/OsCKX2 confers heavy-panicle rice with excellent lodging resistance. J Integr Plant Biol 64:23-38 (2022). PubMed: 34783157
- Qin P et al. Leaf-derived ABA regulates rice seed development via a transporter-mediated and temperature-sensitive mechanism. Sci Adv 7:N/A (2021). PubMed: 33523901
- Zhu Y et al. TERMINAL FLOWER 1-FD complex target genes and competition with FLOWERING LOCUS T. Nat Commun 11:5118 (2020). PubMed: 33046692
- Wang C et al. An AC-Rich Bean Element Serves as an Ethylene-Responsive Element in Arabidopsis. Plants (Basel) 9:N/A (2020). PubMed: 32823972
- Kubo T et al. Two Tightly Linked Genes at the hsa1 Locus Cause Both F1 and F2 Hybrid Sterility in Rice. Mol Plant 9:221-232 (2016). PubMed: 26455463
- Cai Q et al. Jasmonic acid regulates spikelet development in rice. Nat Commun 5:3476 (2014). WB . PubMed: 24647160
- Jasik J et al. Subtissue-specific evaluation of promoter efficiency by quantitative fluorometric assay in laser microdissected tissues of rapeseed. Plant Physiol 157:563-73 (2011). IHC-Fr . PubMed: 21825109