Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end (ab50148)

Overview

  • Product name
    Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end
  • Description
    Rabbit polyclonal to beta Glucuronidase (GUS) - Aminoterminal end
  • Specificity
    ab50148 recognizes bacterial beta Glucuronidase expressed in transgenic tobacco plants.
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Synthetic peptide corresponding to Escherichia coli Beta Glucuronidase (GUS) aa 1-16 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    MLRPVETPTREIKKLD

  • Positive control
    • Cell extract (cytosolic fraction) of leaves from transgenic Nicotiana tabacum expressing E. coli GUS.

Properties

Applications

Our Abpromise guarantee covers the use of ab50148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa. Staining of the beta Glucuronidase band in immunoblotting is specifically inhibited by the immunizing peptide.
IHC-Fr Use at an assay dependent concentration. PubMed: 21825109

Target

  • Relevance
    Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • Beta D glucuronidase antibody
      • Beta glucuronidase antibody
      • gurA antibody
      • UidA antibody

    References

    This product has been referenced in:
    • Cai Q  et al. Jasmonic acid regulates spikelet development in rice. Nat Commun 5:3476 (2014). WB . Read more (PubMed: 24647160) »
    • Jasik J  et al. Subtissue-specific evaluation of promoter efficiency by quantitative fluorometric assay in laser microdissected tissues of rapeseed. Plant Physiol 157:563-73 (2011). IHC-Fr . Read more (PubMed: 21825109) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you very much for your question. Indeed the ab50148 is a polylconal antibody of rabbit origin. The antibody has been purified by affinity to the immunogen and the isotype has not been determined. However the rabbit immune system is only capable o...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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