Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end (ab50148)
- Product nameAnti-beta Glucuronidase (GUS) antibody - Aminoterminal end
- DescriptionRabbit polyclonal to beta Glucuronidase (GUS) - Aminoterminal end
- Specificityab50148 recognizes bacterial beta Glucuronidase expressed in transgenic tobacco plants.
- Tested applicationsWB, IHC-Fr more details
- Species reactivityReacts with: Escherichia coli
Synthetic peptide: mlrpvetptreikkld conjugated to KLH, corresponding to N terminal amino acids 1-16 of Escherichia coli beta Glucuronidase
- Positive controlCell extract (cytosolic fraction) of leaves from transgenic Nicotiana tabacum expressing E. coli GUS.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab50148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa. Staining of the beta Glucuronidase band in immunoblotting is specifically inhibited by the immunizing peptide.|
|IHC-Fr||IHC-Fr: Use at an assay dependent concentration. PubMed: 21825109|
- RelevanceReporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.
- Cellular localizationCytoplasmic
- Beta D glucuronidase antibodyBeta G1 antibodyBeta glucuronidase antibody
- FLJ39445 antibodyGlucuronidase beta antibodygurA antibodyGUSB antibodyMPS 7 antibodyMPS7 antibodyUidA antibody
References for Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end (ab50148)
This product has been referenced in:
- Jasik J et al. Subtissue-specific evaluation of promoter efficiency by quantitative fluorometric assay in laser microdissected tissues of rapeseed. Plant Physiol 157:563-73 (2011). IHC-Fr . Read more (PubMed: 21825109) »