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Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end (ab50148)

Submit an Abreview Q&A (1)Specific References (1)

Overview

  • Product nameAnti-beta Glucuronidase (GUS) antibody - Aminoterminal end
  • Description
    Rabbit polyclonal to beta Glucuronidase (GUS) - Aminoterminal end
  • Specificityab50148 recognizes bacterial beta Glucuronidase expressed in transgenic tobacco plants.
  • Tested applicationsWB, IHC-Fr more details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Synthetic peptide: mlrpvetptreikkld conjugated to KLH, corresponding to N terminal amino acids 1-16 of Escherichia coli beta Glucuronidase

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlCell extract (cytosolic fraction) of leaves from transgenic Nicotiana tabacum expressing E. coli GUS.

Properties

Applications

Our Abpromise guarantee covers the use of ab50148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa. Staining of the beta Glucuronidase band in immunoblotting is specifically inhibited by the immunizing peptide.
IHC-Fr IHC-Fr: Use at an assay dependent concentration. PubMed: 21825109

Target

  • RelevanceReporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.
  • Cellular localizationCytoplasmic
  • Database links
    • Alternative names
        Beta D glucuronidase antibodyBeta G1 antibodyBeta glucuronidase antibody
        FLJ39445 antibodyGlucuronidase beta antibodygurA antibodyGUSB antibodyMPS 7 antibodyMPS7 antibodyUidA antibody
      see all

    References for Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end (ab50148)

    This product has been referenced in:
    • Jasik J  et al. Subtissue-specific evaluation of promoter efficiency by quantitative fluorometric assay in laser microdissected tissues of rapeseed. Plant Physiol 157:563-73 (2011). IHC-Fr . Read more (PubMed: 21825109) »

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    Dear technical support team: This customer raised an enquire about ab50148 (Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end), she wants to know which subclass of this isotype is (Is IgG 1, 2a, 2b, 3 or 4)? Could you please help this customer to...

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    Thank you very much for your question. Indeed the ab50148 is a polylconal antibody of rabbit origin. The antibody has been purified by affinity to the immunogen and the isotype has not been determined. However the rabbit immune system is only capable of p...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"