You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)

Q&A (18)Specific References (1)

Overview

  • Product namebeta Hydroxybutyrate (beta HB) Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.01 mM
  • Range
    0.01 mM - 0.2 mM
  • Assay time
    0h 40m
  • Product overview

    Abcam's beta Hydroxybutyrate (beta HB) Assay Kit utilizes beta HB Dehydrogenase to generate a product which reacts with our colorimetric probe with an absorbance band at 450 nm. The kit is an easy and accurate assay to measure beta HB levels in biological samples. The assay is linear for 1-20 nmol beta HB in up to 100 µl samples or 0.01-0.2 mM of beta HB samples
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Diabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of glucose to the body. The physiological response is for the liver to produce ketone bodies (acetoacetate, acetone, and primarily beta-hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceDiabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of glucose to the body. The physiological response is for the liver to produce ketone bodies (acetoacetate, acetone, and primarily beta hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.
    • Alternative names
        3 hydroxybutyric acidbeta HBbeta Hydroxybutyric acid

    Applications

    Our Abpromise guarantee covers the use of ab83390 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    beta Hydroxybutyrate (beta HB) Assay Kit images

    • Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)
      Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)
      beta HB Standard Curve: Assays were performed following ab83390 kit protocol.

    Protocols

    References for beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)

    This product has been referenced in:
    • van Diepen JA  et al. Caspase-1 deficiency in mice reduces intestinal triglyceride absorption and hepatic triglyceride secretion. J Lipid Res 54:448-56 (2013). Read more (PubMed: 23160218) »

    See 1 Publication for this product

    Publishing research using ab83390 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab83390

    Displaying 1 - 10 of 18 results for Q&A

    10
    Votes: Highest First

    I have order two Abcam kits to measure beta-HB and Ammonia in mouse serum sample--beta Hydroxybutyrate (beta HB) Assay Kit (ab83390) and Ammonia Assay Kit (ab83360). Do you have any special suggestions on how to prepare blood sample?

    For serum or plasma samples, there are no special requirements for preparation of serum or plasma samples for the ammonia assay. For the beta HB assay, it is recommended (see section 4 of the protocol) that samples be de-proteinated with a 10 kDa filter, ...

    Read More

    beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)

    Can we use this kit on post mortem blood samples?

    Thank you for confirming the product number.

    Could you confirm if you are planning on making plasma/serum from the blood samples? If so, yes you can definitely use this kit with plasma and serum. The colour of heme and the red blood cells would...

    Read More

    using the ab93349 assay kit to detect hydroxybutyrate. I understand that the enzyme provided in the kit is the hydroxybutyrate dehydrogenase. In order for the kit to work, is the assumption made that the biological fluid being analyzed has the diaphoras...

    Read More

    The principle you have correct, but the samples need not have any diaphorase. Everything required for this assay to work is being provided in the kit components.

    I hope this information has been useful for you. Please let me know if you have an...

    Read More

    Hello

    I'd like to confirm that your
    beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)
    can be used with plasma (serum) samples that were frozen and stored at -80C. The samples have not undergone any freeze-thaw cycles. Please let me k...

    Read More

    Thank you for your inquiry.
    I can confirm that frozen serum or plasma samples are suitable for this kit.
    We did not experimentally test frozen versus fresh samples but would expect a slight loss of activity in frozen samples.
    This will not ...

    Read More

    Dear Sir/madam,

    I am interested the kit you provides.

    We plane to measure the beta Hydroxybutyrate from tissue.

    In the instruction saying it can be used to measure tissue extract.

    Can I ask you, what kind of the...

    Read More

    Thank you for your enquiry.

    I can confirm that tissue samples in the form of homogonate can be used with this kit.

    In this case, carefully cut up the tissue to small pieces, then lyse using the kit Assay Buffer. To remove interfering...

    Read More

    Do you know if methyl 3 hyroxy butyrate or similar esters will inhibit the beta hydroxybutyrate dehydrogenase in the kit? Thank you,

    Dr. Tanya is out of office this week so I am dealing with her inquiries.

    Unfortunately we haven't done any further tests with this kit so I am sorry we are unable to confirm the suitability of this kit for detection of methyl 3 hyroxy butyrate o...

    Read More

    Hello. Can you tell me if the beta HB assay will also measure HB esters such as methyl 3 hydroxy butyrate or butyrate dimers?

    Thank you,

    Thank you for your enquiry and interest in our products.

    I can confirm that this kit (ab83390: beta Hydroxybutyrate (beta HB) Assay Kit) measuresonly the β-Hydroxybutyrate in the samples.

    Hope this information has been helpful for...

    Read More

    - Can we use XXXX 24kDa cut off filters?
    - In assay protcol step 4 the readings increases after incubation 30 minutes and rises untill 7 hours.
    - Sample used were Drosophila homogenates
    - All the reading were in standard range

    Thank you for contacting us.

    You can certainly use the spin column from XXXXXX however the procedure should be followed as per manufacturer's datasheet.

    Regarding the high readings, this assay has not been standardized for drosophila s...

    Read More

    High background using this kit with fish plasma samples.

    Thank you for your phone call a couple of weeks ago, and I sincerely apologize for the delay getting back to you.

    I've forwarded your data to our assay expert in the lab, and the kit has been standardized using mammalian samples so it may requir...

    Read More

    Inquiry: We are interested in purchasing one assay kit for beta hydroxybutyrate (ab83390). Please give us instructions to place the order from Spain Thank you in advance

    Gracias por contactarnos.

    Puede contactarnos directamente en español, telefónicamente (91 114 65 54), o bien por correo electrónico, a:

    mailto:orders@abcam.com Para tramitar pedidos y otras consultas relacionadas c...

    Read More
    

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"