beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)

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Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)

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Product Name

beta Hydroxybutyrate (beta HB) Assay Kit

Product type

Kits

Description

beta Hydroxybutyrate (beta HB) Assay Kit

Research areas

Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay

Product overview

This beta Hydroxybutyrate (beta HB) Assay Kit utilizes beta HB Dehydrogenase to generate a product which reacts with our colorimetric probe with an absorbance band at 450 nm. The kit is an easy and convenient assay to measure beta HB levels in biological samples. The assay is linear for 1-20 nmol beta HB in up to 100 µl samples or 0.01-0.2 mM of beta HB in samples.

Components

Kit Components	Unit Size/Volume	Cap Colour
beta HB Assay Buffer	25ml	WM
beta HB Enzyme Mix	lyophilised	Green
beta HB Substrate mix	lyophilised	Red
beta HB Standard (1.0 µmol)	lyophilised	Yellow

Note: Please Click Here for Instructions For Use/Protocol

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Tested applications
(see key)

FuncS

Notes

Protect kit from light and moisture. Warm up beta HB Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening.
Keep Enzyme Mix on ice, while in use. Store at –20°C. Stable for at least two months.
Store Substrate Mix at –20°C and protect from light.

Material safety datasheet (MSDS) for this product:
Beta-Hydroxybuterate Assay Kit MSDS

Relevance

Diabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of glucose to the body. The physiological response is for the liver to produce ketone bodies (acetoacetate, acetone, and primarily beta hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.

beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)

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