Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Overview
Product name
beta Hydroxybutyrate (beta HB) Assay Kit
Product overview
This beta Hydroxybutyrate (beta HB) Assay Kit utilizes beta HB Dehydrogenase to generate a product which reacts with our colorimetric probe with an absorbance band at 450 nm. The kit is an easy and convenient assay to measure beta HB levels in biological samples. The assay is linear for 1-20 nmol beta HB in up to 100 µl samples or 0.01-0.2 mM of beta HB in samples.
Notes
Protect kit from light and moisture. Warm up beta HB Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening.
Keep Enzyme Mix on ice, while in use. Store at –20°C. Stable for at least two months.
Store Substrate Mix at –20°C and protect from light.
Keep Enzyme Mix on ice, while in use. Store at –20°C. Stable for at least two months.
Store Substrate Mix at –20°C and protect from light.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
| Components | Identifier | 100 tests |
|---|---|---|
| beta HB Assay Buffer | WM | 1 x 25ml |
| beta HB Enzyme Mix (lyophilised) | Green | 1 vial |
| beta HB Standard (1.0 µmol) (lyophilised) | Yellow | 1 vial |
| beta HB Substrate mix (lyophilised) | Red | 1 vial |
Research areas
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Lipid Metabolism Kits >> Lipid metabolism
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Dehydrogenase Kits
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
-
Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)
(enlarge)
Relevance
Diabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of glucose to the body. The physiological response is for the liver to produce ketone bodies (acetoacetate, acetone, and primarily beta hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.
Alternative names
- 3 hydroxybutyric acid
- beta HB
- beta Hydroxybutyric acid
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution.
beta Hydroxybutyrate (beta HB) Assay Kit images:
Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)
beta HB Standard Curve: Assays were performed following ab83390 kit protocol.
Protocols
References for beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)
ab83390 has not yet been referenced specifically in any publications.
Publishing research using ab83390? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"