Overview

  • Product namebeta Hydroxybutyrate (beta HB) Assay KitSee all Beta Hydroxybutyrate kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.01 mM
  • Range
    0.01 mM - 0.2 mM
  • Assay time
    0h 40m
  • Product overview

    Abcam's beta Hydroxybutyrate (beta HB) Assay Kit utilizes beta HB Dehydrogenase to generate a product which reacts with our colorimetric probe with an absorbance band at 450 nm. The kit is an easy and accurate assay to measure beta HB levels in biological samples. The assay is linear for 1-20 nmol beta HB in up to 100 µl samples or 0.01-0.2 mM of beta HB samples
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Diabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of glucose to the body. The physiological response is for the liver to produce ketone bodies (acetoacetate, acetone, and primarily beta-hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceDiabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of glucose to the body. The physiological response is for the liver to produce ketone bodies (acetoacetate, acetone, and primarily beta hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.
  • Alternative names
    • 3 hydroxybutyric acid
    • beta HB
    • beta Hydroxybutyric acid

Applications

Our Abpromise guarantee covers the use of ab83390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

beta Hydroxybutyrate (beta HB) Assay Kit images

  • beta HB Standard Curve: Assays were performed following ab83390 kit protocol.

Protocols

References for beta Hydroxybutyrate (beta HB) Assay Kit (ab83390)

This product has been referenced in:
  • van Diepen JA  et al. Caspase-1 deficiency in mice reduces intestinal triglyceride absorption and hepatic triglyceride secretion. J Lipid Res 54:448-56 (2013). Read more (PubMed: 23160218) »

See 1 Publication for this product

Product Wall

The detection agent does not quantify the oxobutyrate but the byproduct of the reaction by the dehydrogenase. The reaction byproducts are produced proportionally based on the amount of beta hydroxybutyrate in a specific sample. This is why this assay k...

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Thank you for your enquiry and interest in our products.

I can confirm that this kit (ab83390: beta Hydroxybutyrate (beta HB) Assay Kit) measuresonly the β-Hydroxybutyrate in the samples.

Hope this information has been helpful ...

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For serum or plasma samples, there are no special requirements for preparation of serum or plasma samples for the ammonia assay. For the beta HB assay, it is recommended (see section 4 of the protocol) that samples be de-proteinated with a 10 kDa filte...

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Thank you for confirming the product number.

Could you confirm if you are planning on making plasma/serum from the blood samples? If so, yes you can definitely use this kit with plasma and serum. The colour of heme and the red blood cells wo...

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The principle you have correct, but the samples need not have any diaphorase. Everything required for this assay to work is being provided in the kit components.

I hope this information has been useful for you. Please let me know if you have...

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Thank you for your inquiry.
I can confirm that frozen serum or plasma samples are suitable for this kit.
We did not experimentally test frozen versus fresh samples but would expect a slight loss of activity in frozen samples.
This will n...

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Thank you for your enquiry.

I can confirm that tissue samples in the form of homogonate can be used with this kit.

In this case, carefully cut up the tissue to small pieces, then lyse using the kit Assay Buffer. To remove interfer...

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Thank you for contacting us.

You can certainly use the spin column from XXXXXX however the procedure should be followed as per manufacturer's datasheet.

Regarding the high readings, this assay has not been standardized for drosophil...

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Thank you for your phone call a couple of weeks ago, and I sincerely apologize for the delay getting back to you.

I've forwarded your data to our assay expert in the lab, and the kit has been standardized using mammalian samples so it may req...

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mailto:orders@abcam.com Para tramitar pedidos y otras consultas relacionada...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"