Human beta IG H3 ELISA Kit (TGFBI) (ab155426)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 0.025 ng/ml - 6 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human beta IG H3 ELISA Kit (TGFBI)
See all IGH3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall < 10% Inter-assay Sample n Mean SD CV% overall < 12% -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
< 25 pg/ml -
Range
0.025 ng/ml - 6 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum = 92.44 76% - 113% Plasma = 86.91 69% - 104% Cell culture media = 104.8 96% - 114% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s beta IG H3 (TGFBI) Human ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human beta IG H3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human beta IG H3 (TGFBI) coated on a 96-well plate. Standards and samples are pipetted into the wells and beta IG H3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human beta IG H3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of beta IG H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization maybe required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml beta IG H3 Microplate (12 x 8 wells) 1 unit Biotinylated anti-Human beta IG H3 2 vials Recombinant Human beta IG H3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Cellular localization
Secreted, extracellular space, extracellular matrix. -
Alternative names
- Beta ig-h3
- beta-IG-H3
- BIGH3
see all -
Database links
- Entrez Gene: 7045 Human
- Omim: 601692 Human
- SwissProt: Q15582 Human
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab155426 has been referenced in 5 publications.
- Peng P et al. TGFBI secreted by tumor-associated macrophages promotes glioblastoma stem cell-driven tumor growth via integrin αvβ5-Src-Stat3 signaling. Theranostics 12:4221-4236 (2022). PubMed: 35673564
- Liu J et al. The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33431665
- Bissey PA et al. Dysregulation of the MiR-449b target TGFBI alters the TGFß pathway to induce cisplatin resistance in nasopharyngeal carcinoma. Oncogenesis 7:40 (2018). PubMed: 29795279
- Pan T et al. BIGH3 Promotes Osteolytic Lesions in Renal Cell Carcinoma Bone Metastasis by Inhibiting Osteoblast Differentiation. Neoplasia 20:32-43 (2018). PubMed: 29190493
- Nam SM et al. Ex Vivo Expansion of Human Limbal Epithelial Cells Using Human Placenta-Derived and Umbilical Cord-Derived Mesenchymal Stem Cells. Stem Cells Int 2017:4206187 (2017). PubMed: 28894469