Overview

  • Product nameAnti-beta III Tubulin antibody [TU-20] (FITC)
    See all beta III Tubulin primary antibodies
  • Description
    Mouse monoclonal [TU-20] to beta III Tubulin (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Conjugation notesThe purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
  • SpecificityThis antibody recognizes the peptide sequence ESESQGPK (amino acids 441-448) of human class III beta tubulin specific for neurones.
  • Tested applicationsICC, IHC-FoFrmore details
  • Species reactivity
    All (the recognized epitope is conserved within all species).
  • Immunogen

    Synthetic peptide: corresponding to amino acids (C)441-448 (

    ESESQGPK

    ) of beta III Tubulin conjugated to maleimide activated KLH via cysteine added to the N terminus of the neurone specific peptide.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesSize exclusion chromatography.
  • Clonality Monoclonal
  • Clone numberTU-20
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab25770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/40.
IHC-FoFr Use at an assay dependent dilution.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
  • Tissue specificityExpression is primarily restricted to central and peripheral nervous system.
  • Involvement in diseaseDefects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
  • Sequence similaritiesBelongs to the tubulin family.
  • DomainThe highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Alternative names
    • beta 3 tubulin antibody
    • beta-4 antibody
    • CDCBM antibody
    • CFEOM3A antibody
    • M(beta)3 antibody
    • M(beta)6 antibody
    • MC1R antibody
    • Neuron-specific class III beta-tubulin antibody
    • QccE-11995 antibody
    • QccE-15186 antibody
    • TBB3_HUMAN antibody
    • Tubb 3 antibody
    • TUBB 4 antibody
    • Tubb3 antibody
    • Tubb3 antibody
    • TUBB4 antibody
    • Tubulin beta 3 antibody
    • Tubulin beta 3 chain antibody
    • Tubulin beta 4 antibody
    • Tubulin beta III antibody
    • Tubulin beta-3 chain antibody
    • Tubulin beta-4 chain antibody
    • Tubulin beta-III antibody
    see all

Anti-beta III Tubulin antibody [TU-20] (FITC) images

  • Immunohistochemical analysis of innervated frozen Human internal anal sphincter implanted in mice, staining beta III Tubulin with ab25770.

References for Anti-beta III Tubulin antibody [TU-20] (FITC) (ab25770)

This product has been referenced in:
  • Raghavan S  et al. Successful implantation of bioengineered, intrinsically innervated, human internal anal sphincter. Gastroenterology 141:310-9 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21463628) »
  • Launay S  et al. HtrA1-dependent proteolysis of TGF-beta controls both neuronal maturation and developmental survival. Cell Death Differ 15:1408-16 (2008). IHC-FoFr ; Mouse . Read more (PubMed: 18551132) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"