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Product name
Anti-beta IV Tubulin antibody [ONS.1A6]
See all beta IV Tubulin products (3) ...
Description
Mouse monoclonal [ONS.1A6] to beta IV Tubulin
Specificity
The antibody does not react with other tubulin isotypes.
Tested applications
IHC-P, IHC-Fr, ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Hamster, Cow, Human
Immunogen
Synthetic peptide corresponding to the C-terminal sequence, coupled to BSA.
General notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Ascites fluid.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
ONS.1A6
Isotype
IgG1
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. Use with 10% neutral buffered formalinalcoholic formalin-xylene, paraffin-embedded , or 1-2% glutaraldehyde-4% formaldehyde fixed, LR Gold embedded.
IHC-Fr: Use at an assay dependent dilution. PubMed: 17575139
ELISA: Use at an assay dependent dilution.
WB: 1/400. Detects a band of approximately 55 , 100 kDa.
Target
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Sequence similarities
Belongs to the tubulin family.
Domain
The highly acidic C-terminal region may bind cations such as calcium.
Post-translational
modifications
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP04350
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Beta 4 antibody
- Beta 4 tubulin antibody
- beta 5 antibody
see all
Database links
- Entrez Gene: 10382 Human
- Entrez Gene: 22153 Mouse
- Entrez Gene: 29213 Rat
- Omim: 602662 Human
- SwissProt: P04350 Human
- SwissProt: Q9D6F9 Mouse
- Unigene: 110837 Human
- Unigene: 7420 Mouse
References for Anti-beta IV Tubulin antibody [ONS.1A6] (ab11315)
This product has been referenced in:
- Atochina-Vasserman EN et al. Early Alveolar Epithelial Dysfunction Promotes Lung Inflammation in a Mouse Model of Hermansky Pudlak Syndrome. Am J Respir Crit Care Med : (2011).Read more (PubMed: 21616998) »
- Spicakova T et al. Expression and silencing of the microtubule-associated protein Tau in breast cancer cells. Mol Cancer Ther 9:2970-81 (2010). WB; Human.Read more (PubMed: 21062914) »
See all 6 publications for this product
Publishing research using ab11315? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"