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Anti-beta Sarcoglycan antibody (ab83699)

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Overview

Product name

Anti-beta Sarcoglycan antibody
See all beta Sarcoglycan products (3) ...

Description

Rabbit polyclonal to beta Sarcoglycan

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Cow, Pig

Immunogen

Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (FSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNI KVDGRAIVRGN) of Human beta Sarcoglycan (NP_000223)

FSTDYETHEF HLPSGVKSLN VQKASTERIT SNATSDLNIK VDGRAIVRGN

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human fetal heart lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Other ECM Proteins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Glucosaminoglycans

  • Western blot - beta Sarcoglycan antibody (ab83699)Western blot - beta Sarcoglycan antibody (ab83699) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab83699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/312500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

Cellular localization

Cell membrane; sarcolemma; single-pass type II membrane protein

Alternative names

  • 43 kDa dystrophin associated glycoprotein antibody
  • 43DAG antibody
  • A3b antibody
  • Beta SG antibody
  • LGMD2E antibody
  • SGC antibody
  • SGCB antibody
see all

Database links

Anti-beta Sarcoglycan antibody images:

  Western blot - beta Sarcoglycan antibody (ab83699)

Western blot - beta Sarcoglycan antibody (ab83699)

Anti-beta Sarcoglycan antibody (ab83699) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 35 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)


Gel concentration: 12%

References for Anti-beta Sarcoglycan antibody (ab83699)

ab83699 has not yet been referenced specifically in any publications.

Publishing research using ab83699? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"