Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels
Overview
Product name
Anti-beta Sodium Potassium ATPase antibody [0.T.05]
See all beta Sodium Potassium ATPase products (7) ...
Description
Mouse monoclonal [0.T.05] to beta Sodium Potassium ATPase
Specificity
This antibody is specific for the beta 1 subunit isoform of Sodium Potassium ATPase.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Rat, Dog, Human
Does not react with
Mouse
Immunogen
Purified Sodium Potassium ATPase isolated from membrane fractions of rabbit kidney outer medulla.
Positive control
Rat brain microsomal preparation, rat heart and rat kidney microsomal preparations.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 40% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
0.T.05
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IHC-P
Application notes
Immunohistochemistry (PFA fixed/paraffin embedded): Use at a concentration of 10 µg/ml on 4% paraformaldehyde fixed tissue.
WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 48 kDa. Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Tissue specificity
Found in most tissues.
Sequence similarities
Belongs to the X(+)/potassium ATPases subunit beta family.
Cellular localization
Membrane.
Target information above from: UniProt accessionP05026
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adenosinetriphosphatase antibody
- AT1B1_HUMAN antibody
- ATP 1B antibody
see all
Database links
- Entrez Gene: 403966 Dog
- Entrez Gene: 481 Human
- Entrez Gene: 25650 Rat
- Omim: 182330 Human
- SwissProt: P06583 Dog
- SwissProt: P05026 Human
- SwissProt: P07340 Rat
- Unigene: 291196 Human
see all
References for Anti-beta Sodium Potassium ATPase antibody [0.T.05] (ab33144)
This product has been referenced in:
- Jha S & Dryer SE The beta1 subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface. FEBS Lett 583:3109-14 (2009). WB; Human.Read more (PubMed: 19729011) »
See 1 publication for this product
Publishing research using ab33144? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"