Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels
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Do you have a mouse IgG2a ASCITES isotype avialable to use for ab2873? Also, can you give me a little more info on the purity of the antibody? Thank you
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ANSWER: |
Thank you for your enquiry. ab2873 (Sodium/Potassium ATPase Beta) is not affinity purified but comes as a PBS-diluted ascites containing 0.05% sodium azide. Please do not hesitate to contact me should you require further assistance.
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For ab2871 and ab2873 - Customer would like to know if the epitope is extracellular or intracellular. |
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ANSWER: |
Thank you for your patience. Purified lamb kidney beta sodium/potassium ATPase was used as the immunogen for both ab2871 and ab2873. Further characterization of the epitope region has not yet been performed. Therefore we are unable to determine the location of the epitope.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
IHC image of ab2873 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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