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Anti-beta Tubulin antibody [2-28-33] (ab11311)

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Overview

Product name

Anti-beta Tubulin antibody [2-28-33]
See all beta Tubulin products (19) ...

Description

Mouse monoclonal [2-28-33] to beta Tubulin

Specificity

Ab11311 recognizes the two major beta tubulin isotypes and one of the minor beta-tubulin isotypes of the free-living soil nematode Caenorhabditis elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes, to neuronal axons of the larva of Ciona intestinalis (ascidian), and to tubulin of chicken and mammals (e.g., cultured human fibroblasts, bovine, and rat brain tissue). In C. elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and beta-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels, and found reactive with heat-denatured and reduced mammalian preparations in immunoblotting.

Tested applications

ICC/IF, ICC, IF, WBmore details

Cross reactivity

Reacts with

Chicken, Human, Sea Urchin

Predicted to work with

all Mammals

Immunogen

Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Positive control

Cultured human fibroblasts.

General notes

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

2-28-33

Isotype

IgG1

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Tissue specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Sequence similarities

Belongs to the tubulin family.

Domain

The highly acidic C-terminal region may bind cations such as calcium.

Post-translational
modifications

Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.

Cellular localization

Cytoplasm > cytoskeleton.

Target information above from: UniProt accessionP07437 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Beta 4 tubulin antibody
  • Beta 5 tubulin antibody
  • beta Ib tubulin antibody
  • M40 antibody
  • MGC16435 antibody
  • OK/SW cl.56 antibody
  • OK/SWcl.56 antibody
  • TBB5_HUMAN antibody
  • TUBB 1 antibody
  • TUBB 2 antibody
  • TUBB 5 antibody
  • TUBB antibody
  • TUBB1 antibody
  • TUBB2 antibody
  • TUBB5 antibody
  • tubulin beta 1 chain antibody
  • Tubulin beta 2 chain antibody
  • tubulin beta 5 chai antibody
  • Tubulin beta chain antibody
  • Tubulin beta polypeptide antibody
  • Tubulin beta-2 chain antibody
  • Tubulin beta-5 chain antibody
see all

Database links

References for Anti-beta Tubulin antibody [2-28-33] (ab11311)

This product has been referenced in:

  • Scharadin TMet al. TIG3 Tumor Suppressor-Dependent Organelle Redistribution and Apoptosis in Skin Cancer Cells. PLoS One 6:e23230 (2011). ICC/IF; Human.Read more (PubMed: 21858038) »
  • Jiang Het al. Type I transglutaminase accumulation in the endoplasmic reticulum may be an underlying cause of autosomal recessive congenital ichthyosis. J Biol Chem 285:31634-46 (2010). ICC/IF; Human.Read more (PubMed: 20663883) »

See all 2 publications for this product

Publishing research using ab11311? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"