|
|
|
|
|
Product Name
|
beta Tubulin antibody [2-28-33]
|
| |
See all beta Tubulin antibodies (14)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [2-28-33] to beta Tubulin
|
|
Immunogen
|
Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
|
|
Reacts with
|
Chk, S urc
|
|
Predicted to react
(species key)
|
Mamm
(Due to sequence homology)
|
|
Specificity
|
Ab11311 recognizes the two major beta tubulin isotypes and one of the minor beta-tubulin isotypes of the free-living soil nematode Caenorhabditis elegans.
It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes, to neuronal axons of the larva of Ciona intestinalis (ascidian), and to tubulin of chicken and mammals (e.g., cultured human fibroblasts, bovine, and rat brain tissue).
In C. elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and beta-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels, and found reactive with heat-denatured and reduced mammalian preparations in immunoblotting.
|
|
Tested applications
(see key)
|
ICC/IF, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC: Use at an assay dependent dilution.
IF: 1/1000, this dilution was determined by indirect immunofluorescent labeling of cultured human fibroblasts.
WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Cultured human fibroblasts.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
|
|
Relevance
|
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
2-28-33
|
|
Isotype
|
IgG1
|
|
Purity
|
Ascites
|
|
Storage buffer
|
Preservative: 0.1% Sodium Azide
Constituents: Raw Ascites Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Batch dependent within range: 3.60 - 3.90 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
|
Notes
|
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this beta Tubulin antibody [2-28-33] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to beta Tubulin
|
| |
beta Tubulin antibody [2-28-33] - more information
|
Customer reviews (feedback) regarding beta Tubulin antibody [2-28-33] |
Customer FAQs regarding beta Tubulin antibody [2-28-33] |
Protocols for beta Tubulin antibody [2-28-33] |
Price and availability of products related to beta Tubulin antibody [2-28-33] |
| |
beta Tubulin antibody [2-28-33] - related products:
|
|
|
| |
beta Tubulin antibody [2-28-33] - other tools:
|
Contact Abcam with a Technical Enquiry about beta Tubulin antibody [2-28-33] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
