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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Overview
Product name
Anti-beta Tubulin antibody [D66]
See all beta Tubulin products (19) ...
Description
Mouse monoclonal [D66] to beta Tubulin
Specificity
Ab11307 recognizes all mouse brain beta tubulin isoforms (beta and beta subpopulations), and sea urchin beta 2 tubulin.
Tested applications
ELISA, WB, ICC, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Chicken, Hamster, Cow, Dog, Human, Chlamydomonas reinhardtii, Sea Urchin
Immunogen
Sea urchin (Lytechinus pictus) sperm axonemal proteins.
Epitope
The epitope recognized by the antibody is localized in the C-terminal region of beta tubulin (amino acids 427-432 (sequence DATAEE) in sea urchin, human and mouse beta 2 tubulin) which has been identified as important in flagellar motility.
Positive control
Cultured rat adrenal pheochromocytoma (PC-12) cells.
General notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
D66
Isotype
IgG1
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 0.5 - 1 µg/ml.Predicted molecular weight: 50 kDa.
ICC: Use at an assay dependent dilution. (3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone.)
IP: Use at an assay dependent dilution.
Target
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Tissue specificity
Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Sequence similarities
Belongs to the tubulin family.
Domain
The highly acidic C-terminal region may bind cations such as calcium.
Post-translational
modifications
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP07437
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
see all
Database links
- Entrez Gene: 396254 Chicken
- Entrez Gene: 281555 Cow
- Entrez Gene: 203068 Human
- Entrez Gene: 22153 Mouse
- Entrez Gene: 29214 Rat
- Omim: 191130 Human
- SwissProt: Q148E2 Cow
- SwissProt: P07437 Human
see all
References for Anti-beta Tubulin antibody [D66] (ab11307)
This product has been referenced in:
- Hua Get al. A negative feedback regulatory loop associates the tyrosine kinase receptor ERBB2 and the transcription factor GATA4 in breast cancer cells. Mol Cancer Res 7:402-14 (2009). WB; Human.Read more (PubMed: 19276186) »
See 1 publication for this product
Publishing research using ab11307? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"