Anti-beta Tubulin antibody - Loading Control (ab21057)
Overview
- Product nameAnti-beta Tubulin antibody - Loading ControlSee all beta Tubulin primary antibodies ...
- DescriptionGoat polyclonal to beta Tubulin - Loading Control
- Specificityab21057 detects a band of ~50kDa in Hela and A431 lysates (data for A431 not shown). These bands are blocked by the addition of the immunizing peptide, ab20775.
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Mouse, Human, African Green Monkey
Predicted to work with: Rat, Chicken - Immunogen
Synthetic peptide derived from within residues 1 - 100 of Human beta Tubulin.
(Peptide available as ab20775.)
- Positive controlHeLa whole cell lysate, HeLa nuclear lysate and A431 cell lysate
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
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Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab21057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| ICC/IF |
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa). Can be blocked with beta Tubulin peptide (ab20775).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
- Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
- Sequence similaritiesBelongs to the tubulin family.
- DomainThe highly acidic C-terminal region may bind cations such as calcium.
- Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. - Cellular localizationCytoplasm > cytoskeleton.
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Database links
- Entrez Gene: 396254 Chicken
- Entrez Gene: 203068 Human
- Entrez Gene: 22153 Mouse
- Omim: 191130 Human
- SwissProt: P07437 Human
- SwissProt: Q9D6F9 Mouse
- Unigene: 636480 Human
- Unigene: 706187 Human
- Unigene: 714425 Human
see all
Target information above from: UniProt accession
P07437
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- Beta 4 tubulin antibodyBeta 5 tubulin antibodyBetaTubulin antibody
- dJ40E16.7 antibodyTBB5_HUMAN antibodyTUBB antibodyTUBB2 antibodyTUBB2A antibodyTUBB5 antibodytubulin beta 2A antibodyTubulin beta chain antibodyTubulin beta-5 chain antibody
see all
Anti-beta Tubulin antibody - Loading Control images
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Western blot - beta Tubulin antibody - Loading Control (ab21057)All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate withbeta Tubulin peptide (ab20775) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti Goat polyclonal at 1/10000 dilution
Predicted band size : 50 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - beta Tubulin antibody - Loading Control (ab21057)This image is courtesy of an Abreview submitted by Dr Kirk McManusAb21057 staining beta-tubulin in asynchronous HeLa cells (green). Cells were counterstained with DAPI (red) to show the nucleus. Secondary antibody: Donkey anti-Goat antibody conjugated to Alexafluor 488 ® (Please refer to the Abreview for further details). -
Western blot - Anti-beta Tubulin antibody - Loading Control (ab21057)
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Exposure time : 150 seconds
References for Anti-beta Tubulin antibody - Loading Control (ab21057)
This product has been referenced in:
- Scarlett UK et al. Ovarian cancer progression is controlled by phenotypic changes in dendritic cells. J Exp Med 209:495-506 (2012). WB ; Human . Read more (PubMed: 22351930) »
- Stingele S et al. Global analysis of genome, transcriptome and proteome reveals the response to aneuploidy in human cells. Mol Syst Biol 8:608 (2012). Read more (PubMed: 22968442) »
