Anti-beta Tubulin antibody [TU-06] (ab7792)

Western blot - beta Tubulin antibody [TU-06] (ab7792)

Anti-beta Tubulin antibody [TU-06] (ab7792) at 1/500 dilution + Human U87 MG whole cell lysate with 5% Milk for 1 hour at 22°C.

Secondary
HRP-conjugated Sheep anti-mouse IgG monoclonal at 1/5000 dilution

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence-beta Tubulin antibody [TU-06](ab7792)

ICC/IF image of ab7792 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7792, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

Flow Cytometry-beta Tubulin antibody [TU-06](ab7792)

Overlay histogram showing HeLa cells stained with ab7792 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100® for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7792, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100® used under the same conditions.