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Anti-beta Tubulin antibody [TU-06] (ab78109)

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Overview

  • Product nameAnti-beta Tubulin antibody [TU-06]See all beta Tubulin primary antibodies ...
  • Description
    Mouse monoclonal [TU-06] to beta Tubulin
  • SpecificityRecognizes all beta Tubulin isoforms.
  • Tested applicationsWB, IHC-P, ICC more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Porcine brain beta Tubulin.

  • Positive controlHeart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab78109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
IHC-P IHC-P: Use a concentration of 5 µg/ml.
ICC ICC: Use a concentration of 2 µg/ml.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
  • Sequence similaritiesBelongs to the tubulin family.
  • DomainThe highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localizationCytoplasm > cytoskeleton.

    • Target information above from: UniProt accession P07437 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Beta 4 tubulin antibodyBeta 5 tubulin antibodyBetaTubulin antibody
        dJ40E16.7 antibodyTBB5_HUMAN antibodyTUBB antibodyTUBB2 antibodyTUBB2A antibodyTUBB5 antibodytubulin beta 2A antibodyTubulin beta chain antibodyTubulin beta-5 chain antibody
      see all

    Anti-beta Tubulin antibody [TU-06] images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Tubulin antibody [TU-06] (ab78109)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Tubulin antibody [TU-06] (ab78109)
      Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human heart tissue using 5µg/ml ab78109.

      A after heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

    References for Anti-beta Tubulin antibody [TU-06] (ab78109)

    ab78109 has not yet been referenced specifically in any publications.

    Publishing research using ab78109 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab78109 Submit a Question for ab78109

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    Inquiry: Hello, Do you have a beta-tubulin antibody or another specific neuronal loading control that will work under non-reduced conditions? Thank you very much,

    Thank you for contacting Abcam.

    In general, most of our antibodies work under reduced, denatured conditions for western blot. If you desire an antibody that is more likely to work under non-reduced conditions, I recommend choosing an antibody tha...

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    Please find the attached proteins sequences for beta tubulin in T.brucei. If you find any antibody that might cross react kindly let me know.

    Thank you for contacting us.

    The homology of the human immunogen peptide for ab6046 andab21057 is 84%. These antibodies are polyclonal.
    For ab78109, which was raised against the pig protein, the homology of the full length protein is 86%. F...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"