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Product Name
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beta Tubulin antibody [TUB 2.1] (Cy3 ®)
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See all beta Tubulin antibodies (14)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [TUB 2.1] to beta Tubulin (Cy3 ®)
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Immunogen
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Purified rat brain tubulin.
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Hm, Rb
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Specificity
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It reacts with the chymotryptic beta-Lc and beta-Sc tubulin fragments and does not seem to exhibit mouse brain beta-tubulin isoform-related specificity
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Epitope
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Ab11309 specifically recognizes an epitope in the carboxy-terminal part of beta-tubulin (between amino acids 281-446).
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Tested applications
(see key)
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ICC/IF
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/100, this dilution was determined by direct immunofluorescent labeling of cultured chicken fibroblasts or BHK cells. The conjugate may be used in double labeling experiments with fluorescein tagged antibodies.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Cultured chicken fibroblasts or BHK cells.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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Relevance
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Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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TUB 2.1
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Isotype
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IgG1
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Conjugation notes
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The product is prepared by conjugation of Cy3 to purified monoclonal anti-beta-tubulin antibody. The conjugate is purified by gel filtration to remove unbound Cy3 fluorophore.
F/P Molar Ratio: (Cy3:Ab) 3 to 9
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01% PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Conjugation
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Cy3 ®
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Concentration
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Batch dependent within range: 1.00 - 1.20 mg/ml
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Storage instructions
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Store at +4°C in the dark. Do not freeze.
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Notes
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If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this beta Tubulin antibody [TUB 2.1] (Cy3 ®) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to beta Tubulin
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beta Tubulin antibody [TUB 2.1] (Cy3 ®) - more information
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