Anti-beta Tubulin antibody (ab15568)

Our species is yeast(http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae) and we do reduce, denature the proteins.  

ANSWER:

Thank you for your response.

We have predicted cross reactivity with this species, but we have not yet tested in Saccharomyces cerevisiae and did not know what results would occur when using this in yeast. While products used in 'predicted to react' species are not covered by our Abpromise, I can offer you a retroactive 100% Abreview Testing Discount.For UNTESTED species and/or applications, we have established this testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested.

To redeem this code please submit and Abreview for this product in yeast. Please leave this code in the 'additional notes' box. After submitting the Abreview the code will become active, entitling you to one free antibody.

The terms and conditions applicable to this offer can be found here: http://www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards! http://www.abcam.com/abreviews

Thanks for the response. Actually these blots were fresh and were used only with ab15568(Tubilin from Abcam)   Brief protocol   Gel used                               :              hand cast Tris-HCL 10% Gel condition                     :               100V until ladder reached stacking, then shooted it to 200v until dye past the glass @ Room Temperature Transfer                               :               75V 1hr, cold room(4 degree) Blocking                               :               5% milk Room Temperature 2 hrs Primary antibody             :               ab15568 @ 1:1000, in 1% milk Overnight cold room Secondary antibody        :               Rabbit 1:15000 in 1% milk 45 min Room Temperature   Used ECl kit and developed the films.   Also tried the same protocol with ab15568 @ 1:1500 dilution but both the times I am getting strong 3 bands for the control. I am wondering, why I am seeing GAPDH band so strong when I use Tubilin antibody. All are fresh membranes no stripping at all.   Hope this clarifies and your help is appreciated.

ANSWER:

Thank you for that information.

I have just a few more questions regarding this. What species are you testing this product against? Do you run these westerns natively or in a reduced and denatured condition?

If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, as you are using it according to specifications listed on our datasheet.

I look forward to your response. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Dear Tech support

One of our customers is interested in testing ab15568 for WB in two fungi species.

The product's datasheet states the antibody is predicted to react with different Fungi species and the customer wishes to test it for Sclerotinia and Neurospora.

Is it possible to test the antibody for above purpose as part of the abreview promotion.

Please advise.

Thank you for your kind help,

ANSWER:

Thank you for your enquiry.

I can confirm that our source has kindly compared the immunogen for ab15568 against the beta tubulin sequence for Sclerotinia (A7EH60) and Neurospora (P05220) and the percentage homology is 92% and 100%, respectively.

These alignments are high, and we would therefore suggest that the antibody is likely to detect in these fungi species. However, as you are aware I am sorry we are not able to guarantee this without further testing. I would be pleased to provide a discount code for the customer. In order to do so, I would appreciate if you could please provide the customers name and also the name of the institution they are working at for our records and so we can trace the Abreview when it is submitted.

Thank you for your cooperation. I look forward to hearing from you.