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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Overview
Product name
Anti-beta Tubulin antibody
See all beta Tubulin products (19) ...
Description
Chicken polyclonal to beta Tubulin
Tested applications
Cross reactivity
Reacts with
Cow
Immunogen
Full length beta Tubulin (Cow)
Positive control
293 cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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Western blot - beta Tubulin antibody (ab37776)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Target
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Tissue specificity
Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Sequence similarities
Belongs to the tubulin family.
Domain
The highly acidic C-terminal region may bind cations such as calcium.
Post-translational
modifications
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP07437
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
see all
Database links
Anti-beta Tubulin antibody images:
Western blot - beta Tubulin antibody (ab37776)
Anti-beta Tubulin antibody (ab37776) at 1/1000 dilution + 293 cell lysate at 0.5 µg
Secondary
HRP conjugated Goat anti IgY at 1/1000 dilution
Predicted band size : 50 kDa
Observed band size : 50 kDa
References for Anti-beta Tubulin antibody (ab37776)
ab37776 has not yet been referenced specifically in any publications.
Publishing research using ab37776? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"