Overview

  • Product nameAnti-beta Actin [AC-15] antibody (HRP)See all beta Actin primary antibodies ...
  • Description
    Mouse monoclonal [AC-15] to beta Actin (HRP)
  • ConjugationHRP
  • SpecificityThis antibody labels specifically ß-actin in a wide variety of tissues and species. It does not stain adult cardiac and skeletal muscles except for contaminants.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Carp, Monkey
    Predicted to work with: a wide range of other species

    Does not react with

    Fruit fly (Drosophila melanogaster), Dictyostelium discoideum
  • Immunogen

    Synthetic N terminal peptide: DDDIAALVIDNGSGK conjugated to KLH, corresponding 100% to a sequence from: Xenopus borealis (Kenyan clawed frog), Lytechinus pictus (Painted sea urchin) and Engystomops pustulosus (Tungara frog).

  • Positive control
    • Chicken gizzard tissue cryosections. Human foreskin fibroblasts. Chicken fibroblasts. Rat dorsal root ganglion and Rat liver lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab49900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/25000 - 1/50000. Predicted molecular weight: 42 kDa.

Target

Anti-beta Actin [AC-15] antibody (HRP) images

  • All lanes : Anti-beta Actin [AC-15] antibody (HRP) (ab49900) at 1/50000 dilution

    Lane 1 : A549 Lysate
    Lane 2 : HeLa Lysate
    Lane 3 : 3T3 Lysate
    Lane 4 : NRK Lysate
    Lane 5 : MDCK Lysate
    Lane 6 : MDBK Lysate
    Lane 7 : BHK Lysate
    Lane 8 : COS7 Lysate
    Lane 9 : CFB Lysate

    developed using the ECL technique

    Predicted band size : 42 kDa
  • Anti-beta Actin [AC-15] antibody (HRP) (ab49900) at 1/25000 dilution + Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 22°C.

    See Abreview

References for Anti-beta Actin [AC-15] antibody (HRP) (ab49900)

This product has been referenced in:
  • Allen-Petersen BL  et al. Protein kinase Cd is required for ErbB2-driven mammary gland tumorigenesis and negatively correlates with prognosis in human breast cancer. Oncogene N/A:N/A (2013). Mouse . Read more (PubMed: 23474764) »
  • Horan KA  et al. Proteasomal degradation of herpes simplex virus capsids in macrophages releases DNA to the cytosol for recognition by DNA sensors. J Immunol 190:2311-9 (2013). Read more (PubMed: 23345332) »

See all 21 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Jan 08 2014

Application Western blot
Sample Rat Cell lysate - whole cell (ParC5 rat parotid cell line)
Loading amount 50 µg
Specification ParC5 rat parotid cell line
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
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Submitted Sep 28 2010

Application Western blot
Sample Human Cell lysate - whole cell (THP-1)
Loading amount 10 µg
Specification THP-1
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Mar 19 2010

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount 30 µg
Specification Cos-7 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 20 2007

Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount 30 µg
Specification 3T3 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 20 2007

Application Western blot
Sample Hamster Cell lysate - whole cell (CHO cell line)
Loading amount 30 µg
Specification CHO cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 20 2007

Application Western blot
Sample Rat Cell lysate - whole cell (PC12)
Loading amount 30 µg
Specification PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jun 21 2007

Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 20 µg
Specification 293T
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jun 15 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"