Overview

  • Product nameAnti-beta Actin antibody [4C2]
    See all beta Actin primary antibodies
  • Description
    Mouse monoclonal [4C2] to beta Actin
  • SpecificityThe epitope recognized by 4C2 is highly conserved and the antibody is therefore expected to react with many species. ab123020 does not cross react with other actin isoforms.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Pig
    Predicted to work with: a wide range of other speciesDoes not react with: Zebrafish
  • Immunogen

    Synthetic peptide:

    Ac-DDDIAALVV-COOH

    conjugated to KLH through the cysteine residue, corresponding to N terminal amino acids 2-10 of Human beta Actin.

  • Positive control
    • Human dermal fibroblast cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab123020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/100 - 1/500.
IHC-Fr 1/100 - 1/500.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

Target

Anti-beta Actin antibody [4C2] images

  • Overlay histogram showing HeLa cells stained with ab123020 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab123020, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ab123020 at 1/500 dilution staining beta Actin in methanol-fixed Human dermal fibroblasts by Immunofluorescence.
  • Immunofluorescence staining of 3 days old zebrafish embryo
  • Immunofluorescence staining of 1 month old zebrafish embryo

References for Anti-beta Actin antibody [4C2] (ab123020)

This product has been referenced in:
  • Scott DW  et al. Heterogenic endothelial responses to inflammation: role for differential N-glycosylation and vascular bed of origin. J Am Heart Assoc 2:e000263 (2013). WB ; Human . Read more (PubMed: 23900214) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"