Overview

  • Product name
  • Description
    Rabbit polyclonal to beta Actin
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human beta Actin aa 26-33.
    Sequence:

    APRAVFPS


    Database link: P60709

  • Positive control
    • WB: U-2 OS and SK-N-SH cell lysate. IHC-P: Human leiomyoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab16039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP Use a concentration of 5 µg/ml.

Target

Anti-beta Actin antibody images

  • All lanes : Anti-beta Actin antibody (ab16039) at 1 µg/ml

    Lane 1 : SK-N-SH (human neuroblastoma) whole cell lysate
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : U-2 OS (human osteosarcoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • Formalin-fixed, paraffin-embedded human leiomyoma tissue stained for beta Actin with ab16039 (1/100 dilution) in immunohistochemical analysis.

  • beta Actin was immunoprecipitated using 0.5mg SK-N-SH (human neuroblastoma cell line) whole cell extract, 5 µg of Rabbit polyclonal to beta Actin and 50 µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10 minutes, SK-N-SH whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.

    Proteins were eluted by addition of 40 µl SDS loading buffer and incubated for 10 minutes at 70°C; 10 µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16039.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 42kDa; beta Actin

References for Anti-beta Actin antibody (ab16039)

This product has been referenced in:
  • Ding X  et al. KLF7 overexpression in human oral squamous cell carcinoma promotes migration and epithelial-mesenchymal transition. Oncol Lett 13:2281-2289 (2017). WB ; Human . Read more (PubMed: 28454392) »
  • Kong LY  et al. In vivo and in vitro effects of microRNA-27a on proliferation, migration and invasion of breast cancer cells through targeting of SFRP1 gene via Wnt/ß-catenin signaling pathway. Oncotarget 8:15507-15519 (2017). WB ; Human . Read more (PubMed: 28099945) »

See all 24 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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