Our Abpromise guarantee covers the use of ab34731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.
ELISA 1/10000 - 1/50000.
WB 1/500 - 1/3000. Detects a band of approximately 42 kDa.


  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Alternative names
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody images

  • All lanes : Anti-beta Actin antibody (ab34731) at 1/1500 dilution

    Lane 2 : Human embryonic kidney 293
    Lane 3 : Human lung carcinoma A549
    Lane 4 : Mouse brain

    Lysates/proteins at 35 µg per lane.

    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining using ab34731 antibody at 1/200 dilution shows detection of actin at the neuromuscular juntion of rana pipiens tissue fixed in 4% paraformaldehyde using a section 4.2 µm thick.

References for Anti-beta Actin antibody (ab34731)

This product has been referenced in:
  • Zhong T  et al. Repression of contexual fear memory induced by isoflurane is accompanied by reduction in histone acetylation and rescued by sodium butyrate. Br J Anaesth N/A:N/A (2014). Mouse . Read more (PubMed: 24838805) »
  • El Hage A  et al. Genome-wide distribution of RNA-DNA hybrids identifies RNase H targets in tRNA genes, retrotransposons and mitochondria. PLoS Genet 10:e1004716 (2014). WB . Read more (PubMed: 25357144) »

See all 2 Publications for this product

Product Wall

Thank you for your reply.

A replacement vial of ab34731 has been added to Cedarlane's next order. Please do not hesitate to contact us if you have any additional questions or concerns.

Thank you for your reply.

In order to process your request for a replacement, can you please provide your order number for this product? Did you purchase this directly from Abcam or through Cedarlane?

Thank you for contacting Abcam regarding ab34731.

I am sorry that you have been experiencing difficulties with this antibody in your experiments. In order to assist you further, can you please provide some additional information regarding...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Mouse Recombinant protein (Recombinant ActB)
Specification Recombinant ActB
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Type Indirect

Abcam user community

Verified customer

Submitted Apr 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Loading amount 5 µg
Specification MEF
Gel Running Conditions Reduced Denaturing (4-12% Nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 24 2009