Anti-beta Actin antibody [AC-15] (FITC) (ab6277)

Overview

  • Product name
    Anti-beta Actin antibody [AC-15] (FITC)
    See all beta Actin primary antibodies
  • Description
    Mouse monoclonal [AC-15] to beta Actin (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: ICC/IF, ICC, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Drosophila melanogaster, Fish
  • Immunogen

    Synthetic peptide:

    DDDIAALVIDNGSGL

    conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).

  • Epitope
    The N-terminal of the beta isoform of actin
  • Positive control
    • Cultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd.
  • General notes
    Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.


    Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells

Properties

Applications

Our Abpromise guarantee covers the use of ab6277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent concentration. PubMed: 21562077
IHC-FoFr Use at an assay dependent dilution. PubMed: 21445349

Target

Images

  • ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence. 

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x20

  • Immunocytochemistry/ Immunofluorescence analysis of chicken fibroblast cells labeling beta Actin with ab6277 at 1/300 dilution. The cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1/300 Anti-beta Actin antibody [AC-15] (FITC) (ab6277).

     

  • ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x40 no deconvolution.

Protocols

References

This product has been referenced in:
  • Llewellyn KJ  et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB . Read more (PubMed: 26134519) »
  • Perrin BJ  et al. ß-Actin and fascin-2 cooperate to maintain stereocilia length. J Neurosci 33:8114-21 (2013). Mouse . Read more (PubMed: 23658152) »

See all 13 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Lung)
Permeabilization
Yes - 0.1% Triton X
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Jim Hsiao

Verified customer

Submitted Oct 31 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuroglioma cells)
Gel Running Conditions
Reduced Denaturing (12%Acrylamide SDSPAGE)
Loading amount
100 µg
Treatment
0.5%triton
Specification
neuroglioma cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Samira Acajjaoui

Verified customer

Submitted Aug 29 2016

This antibody has not yet been tested for use with on formalin fixed, paraffin sections of mouse tissue.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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