• Product nameAnti-beta Actin antibody [BA3R]
    See all beta Actin primary antibodies
  • Description
    Mouse monoclonal [BA3R] to beta Actin
  • Specificityab125248 recognizes native and denatured forms of beta Actin.
  • Tested applicationsSuitable for: Flow Cyt, WB, ELISA, Dot Blot, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human
  • Immunogen

    Synthetic peptide conjugated to KLH from the N terminal region of beta Actin.

  • Positive control
    • Human, Mouse, Rat and Rabbit tissue lysates
  • General notesab125248 can be stored at 4°C (add 0.05% NaN3) for several days to weeks.



Our Abpromise guarantee covers the use of ab125248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent concentration.
Dot Blot Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.


  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody [BA3R] images

  • beta Actin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to beta Actin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab125248.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 42kDa; beta Actin

  • Overlay histogram showing HeLa cells stained with ab125248 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125248, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • All lanes : Anti-beta Actin antibody [BA3R] (ab125248) at 1/1000 dilution

    Lane 1 : Human tissue lysate
    Lane 2 : Mouse tissue lysate
    Lane 3 : Rat tissue lysate
    Lane 4 : Rabbit tissue lysate

    Lysates/proteins at 20 µg per lane.

    developed using the ECL technique

    Predicted band size : 42 kDa

References for Anti-beta Actin antibody [BA3R] (ab125248)

This product has been referenced in:
  • Metodiev MD  et al. Mutations in the tricarboxylic acid cycle enzyme, aconitase 2, cause either isolated or syndromic optic neuropathy with encephalopathy and cerebellar atrophy. J Med Genet 51:834-8 (2014). Read more (PubMed: 25351951) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab125248.
Please use the links above to contact us or submit feedback about this product.