Anti-beta Actin antibody - Loading Control (ab13822)

Overview

  • Product nameAnti-beta Actin antibody - Loading Control
    See all beta Actin primary antibodies
  • Description
    Chicken polyclonal to beta Actin - Loading Control
  • SpecificityDetects a single clean band at 40kD representing beta actin. Does not cross-react with adult cardiac, smooth or skeletal muscle actin.
  • Tested applicationsIHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chinese Hamster
    Predicted to work with: Rabbit, Chicken, Cow, Dog, Xenopus laevis, Fish
  • Immunogen

    Synthetic peptide derived from residues 1 - 100 of Human beta Actin.

    .

  • Positive control
    • This antibody detects beta actin in HeLa cells in WB and IF This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon.

Properties

Applications

Our Abpromise guarantee covers the use of ab13822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).Can be blocked with Human beta Actin peptide (ab13772).

Target

Anti-beta Actin antibody - Loading Control images

  • All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : CHO-K1 cell lysate Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa


    Exposure time : 90 seconds
  • ICC/IF image of ab13822 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab13822, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-chicken IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • IHC image of beta Actin staining in THuman normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab13822, 10µg/ml overnight at +4°C. A goat-anti chicken HRP secondary antibody  (Ab6877, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References for Anti-beta Actin antibody - Loading Control (ab13822)

This product has been referenced in:
  • Peng X  et al. Cellular plasticity induced by anti-a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor encephalitis antibodies. Ann Neurol 77:381-98 (2015). WB . Read more (PubMed: 25369168) »
  • Xu Y  et al. Expression, functional, and structural analysis of proteins critical for otoconia development. Dev Dyn 239:2659-73 (2010). WB ; Mouse . Read more (PubMed: 20803598) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification Hippocampal homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 13 2014

Application Western blot
Sample Rabbit Cell lysate - whole cell (corneal stromal cells)
Loading amount 10 µg
Specification corneal stromal cells
Gel Running Conditions Reduced Denaturing (4-12% gel)
Blocking step milk diluent (KPL) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Submitted Feb 01 2013

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Application Western blot
Sample Human Cell lysate - whole cell (HUVEC)
Loading amount 10 µg
Specification HUVEC
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step milk diluent (KPL) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Submitted Jan 29 2013

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Application Western blot
Sample Mouse Cell lysate - whole cell (Adipocyte)
Loading amount 5 µg
Specification Adipocyte
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 25 2011

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Application Western blot
Sample Human Cell lysate - whole cell (human fibroblast)
Loading amount 5 µg
Specification human fibroblast
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 09 2008

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Application Western blot
Sample Rat Cell lysate - whole cell (Neonatal Cardiomyocytes)
Loading amount 20 µg
Specification Neonatal Cardiomyocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Jun 07 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"