Anti-beta Actin antibody - Loading Control (ab13822)

Overview

  • Product nameAnti-beta Actin antibody - Loading Control
    See all beta Actin primary antibodies
  • Description
    Chicken polyclonal to beta Actin - Loading Control
  • SpecificityDetects a single clean band at 40kD representing beta actin. Does not cross-react with adult cardiac, smooth or skeletal muscle actin.
  • Tested applicationsIHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chinese Hamster
    Predicted to work with: Rabbit, Chicken, Cow, Dog, Xenopus laevis, Fish
  • Immunogen

    Synthetic peptide derived from residues 1 - 100 of Human beta Actin.

    .

  • Positive control
    • This antibody detects beta actin in HeLa cells in WB and IF This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon.

Properties

Applications

Our Abpromise guarantee covers the use of ab13822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).Can be blocked with Human beta Actin peptide (ab13772).

Target

Anti-beta Actin antibody - Loading Control images

  • All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : CHO-K1 cell lysate Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa


    Exposure time : 90 seconds
  • ICC/IF image of ab13822 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab13822, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-chicken IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • IHC image of beta Actin staining in THuman normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab13822, 10µg/ml overnight at +4°C. A goat-anti chicken HRP secondary antibody  (Ab6877, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References for Anti-beta Actin antibody - Loading Control (ab13822)

This product has been referenced in:
  • Xu Y  et al. Expression, functional, and structural analysis of proteins critical for otoconia development. Dev Dyn 239:2659-73 (2010). WB ; Mouse . Read more (PubMed: 20803598) »
  • Jackson TC  et al. Regional hippocampal differences in AKT survival signaling across the lifespan: implications for CA1 vulnerability with aging. Cell Death Differ 16:439-48 (2009). WB ; Rat . Read more (PubMed: 19039330) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification Hippocampal homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 13 2014

Application Western blot
Sample Rabbit Cell lysate - whole cell (corneal stromal cells)
Loading amount 10 µg
Specification corneal stromal cells
Gel Running Conditions Reduced Denaturing (4-12% gel)
Blocking step milk diluent (KPL) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Submitted Feb 01 2013

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Application Western blot
Sample Human Cell lysate - whole cell (HUVEC)
Loading amount 10 µg
Specification HUVEC
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step milk diluent (KPL) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Submitted Jan 29 2013

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Application Western blot
Sample Mouse Cell lysate - whole cell (Adipocyte)
Loading amount 5 µg
Specification Adipocyte
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 25 2011

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Application Western blot
Sample Human Cell lysate - whole cell (human fibroblast)
Loading amount 5 µg
Specification human fibroblast
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 09 2008

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Application Western blot
Sample Rat Cell lysate - whole cell (Neonatal Cardiomyocytes)
Loading amount 20 µg
Specification Neonatal Cardiomyocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Jun 07 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"