Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) (ab20272)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab20272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772). Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details.

Target

Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) images

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) (ab20272) at 1/5000 dilution

    Lane 1 : human testis lysate
    Lane 2 : human brain lysate
    Lane 3 : human tonsil lysate
    Lane 4 : mouse brain lysate
    Lane 5 : mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa


    Exposure time : 2 minutes
  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) (ab20272) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 3T3 whole cell lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 30 seconds
  • Lanes 1 - 4 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) (ab20272) at 1/5000 dilution (Blocked in 5% BSA)
    Lanes 5 - 8 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) (ab20272) at 1/5000 dilution (Blocked in 5% MILK)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
    Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 1 minute

References for Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) (ab20272)

This product has been referenced in:
  • Fan C  et al. Zbed3 contributes to malignant phenotype of lung cancer via regulating ß-catenin and P120-catenin 1. Mol Carcinog 54 Suppl 1:E138-47 (2015). Read more (PubMed: 25263389) »
  • Fan C  et al. Btbd7 contributes to reduced E-cadherin expression and predicts poor prognosis in non-small cell lung cancer. BMC Cancer 14:704 (2014). WB ; Human . Read more (PubMed: 25253020) »

See all 41 Publications for this product

Product Wall

Thank you for your reply.

I think that sounds like a good choice. I have arranged for the antibody (ab34601) to be sent to you free of charge. This should be with you tomorrow. This is on the order number xxxx(Purchase order number xxxxx). Read More

Thank you for getting back to me. Yes, I consider heating necessary for successful denaturation and reduction of the samples. We routinely do this in our lab and have for the testing of this antibody. I would also recommend the verification of your...

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Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (fly heart tube)
Specification fly heart tube
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Jan 06 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (h9c2)
Specification h9c2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Jan 02 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (MEF)
Specification MEF
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Jan 02 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (hek293)
Specification hek293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

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Submitted Jan 02 2014

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

That appears to be a very old lot number (2008 - 2009). Do you know when you purchased the product? I'm showing most of the...

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Thank you for confirming those details. I think I have tracked down your order. We have one for the 12th of September 2012 delivered to the following address:
xxxxx

The order number was xxxx(purchase order number xxxxx), could this be you...

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Thank you for contacting us yesterday in regards to the Anti-beta Actin antibody [mAbcam8226] (HRP) - Loading Control (ab20272).

I have looked into this case a little further. As we discussed over the phone, the reactivity with Saccharomyces ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 40 µg
Specification liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Sep 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"