Overview

  • Product nameAnti-beta Actin antibody [SP124]
    See all beta Actin primary antibodies
  • Description
    Rabbit monoclonal [SP124] to beta Actin
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide near the N-terminus of Human beta Actin.

  • Positive control
    • Raji cell lystate, Human Placenta tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 42 kDa.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Anti-beta Actin antibody [SP124] images

  • Flow cytometric analysis of rabbit anti-beta Actin (SP124) antibody ab115777 (1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue)

  • Anti-beta Actin antibody [SP124] (ab115777) at 1/200 dilution + Raji cell lystate

    Predicted band size : 42 kDa
  • Staining of Human beta Actin in a Formalin/PFA-fixed paraffin-embedded section of Human Placenta using ab115777 at a dilution of 1/200.

References for Anti-beta Actin antibody [SP124] (ab115777)

This product has been referenced in:
  • Cai SD  et al. MicroRNA-144 inhibits migration and proliferation in rectal cancer by downregulating ROCK-1. Mol Med Rep 12:7396-402 (2015). Read more (PubMed: 26458302) »
  • Navarro-Tableros V  et al. Recellularization of rat liver scaffolds by human liver stem cells. Tissue Eng Part A 21:1929-39 (2015). IHC-Fr ; Human . Read more (PubMed: 25794768) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"