Anti-beta Actin (phospho Y55 + Y53) antibody (ab59381)

Overview

  • Product nameAnti-beta Actin (phospho Y55 + Y53) antibodySee all beta Actin primary antibodies ...
  • Description
    Rabbit polyclonal to beta Actin (phospho Y55 + Y53)
  • SpecificityThis antibody detects endogenous levels of beta Actin only when phosphorylated at tyrosine 53/55.
  • Tested applicationsELISA, IHC-P, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized phosphopeptide derived from human beta Actin around the phosphorylation site of tyrosine 53/55.

  • Positive control
    • Human skeletal muscle tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab59381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/5000.
IHC-P Use at an assay dependent dilution.
WB Use at an assay dependent concentration.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin cytoplasmic 1 antibody
    • Actx antibody
    • beta cytoskeletal actin antibody
    • Beta-actin antibody
    • E430023M04Rik antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin (phospho Y55 + Y53) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using ab59381 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with phosphopeptide.

References for Anti-beta Actin (phospho Y55 + Y53) antibody (ab59381)

ab59381 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (glioblastoma cells)
Loading amount 50 µg
Specification glioblastoma cells
Gel Running Conditions Non-reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Submitted Dec 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"