Anti-beta B1 Crystallin antibody (ab54655)

Overview

  • Product nameAnti-beta B1 Crystallin antibody
    See all beta B1 Crystallin primary antibodies
  • Description
    Mouse monoclonal to beta B1 Crystallin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-253 of Human beta B1 Crystallin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCrystallins are the dominant structural components of the vertebrate eye lens.
    • Involvement in diseaseDefects in CRYBB1 are the cause of cataract congenital nuclear autosomal recessive type 3 (CATCN3) [MIM:611544]. A congenital cataract affecting the central nucleus of the eye. Nuclear cataracts are often not highly visually significant. The density of the opacities varies greatly from fine dots to a dense, white and chalk-like, central cataract. The condition is usually bilateral. Nuclear cataracts are often combined with opacified cortical fibers encircling the nuclear opacity, which are referred to as cortical riders.
      Defects in CRYBB1 are a cause of cataract-microcornea syndrome (CAMIS) [MIM:116150]. An ocular disorder characterized by the association of congenital cataract and microcornea without any other systemic anomaly or dysmorphism. Clinical findings include a corneal diameter inferior to 10 mm in both meridians in an otherwise normal eye, and an inherited cataract, which is most often bilateral posterior polar with opacification in the lens periphery. The cataract progresses to form a total cataract after visual maturity has been achieved, requiring cataract extraction in the first to third decade of life. Microcornea-cataract syndrome can be associated with other rare ocular manifestations, including myopia, iris coloboma, sclerocornea and Peters anomaly. Transmission is in most cases autosomal dominant, but cases of autosomal recessive transmission have recently been described.
    • Sequence similaritiesBelongs to the beta/gamma-crystallin family.
      Contains 4 beta/gamma crystallin 'Greek key' domains.
    • DomainHas a two-domain beta-structure, folded into four very similar Greek key motifs.
    • Post-translational
      modifications
      Specific cleavages in the N-terminal arm occur during lens maturation and give rise to truncated forms, leading to impaired oligomerization and protein insolubilization.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta crystallin B1 antibody
      • Beta-B1 crystallin antibody
      • Beta-crystallin B1 antibody
      • CATCN3 antibody
      • CRBB1_HUMAN antibody
      • CRYBB 1 antibody
      • Crybb1 antibody
      • Crystallin beta B1 antibody
      • CTRCT17 antibody
      • Eye lens structural protein antibody
      • OTTHUMP00000028719 antibody
      see all

    Anti-beta B1 Crystallin antibody images

    • Western blot against tagged recombinant protein immunogen using ab54655 beta B1 Crystallin antibody at 1ug/ml. Predicted band size of immunogen is 54 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    • All lanes : Anti-beta B1 Crystallin antibody (ab54655) at 1/100 dilution

      Lane 1 : Zebrafish lens homogenate
      Lane 2 : Zebrafish lens homogenate
      Lane 4 : Human Beta B1 crystallin
      Lane 5 : Human Beta B2 crystallin (Negative Control)

      Lysates/proteins at 1 µg per lane.

      Secondary
      Alkaline phosphatase-conjugated Goat anti-mouse IgG monoclonal at 1/3000 dilution

      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 30 kDa,82 kDa. We are unsure as to the identity of these extra bands.

      This image is courtesy of an Abreview submitted by Dr. Leonel Trujillo

      See Abreview

    References for Anti-beta B1 Crystallin antibody (ab54655)

    ab54655 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Recombinant protein (lens)
    Loading amount 1 µg
    Specification lens
    Gel Running Conditions Non-reduced Denaturing (10%)
    Blocking step SuperBlock Blocking Buffer-Blotting in TBS as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
    Username

    Leonel Trujillo

    Verified customer

    Submitted Apr 15 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"