Overview

  • Product name
  • Description
    Rabbit polyclonal to beta Casein
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 32-62 of Human beta Casein (NP_001882.1).

  • Positive control
    • MDA-MB435 cell line lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell line: T24/83

Properties

Applications

Our Abpromise guarantee covers the use of ab112595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 25 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Important role in determination of the surface properties of the casein micelles.
  • Tissue specificity
    Mammary gland specific. Secreted in milk.
  • Sequence similarities
    Belongs to the beta-casein family.
  • Post-translational
    modifications
    Form 1-P is phosphorylated once; half of the molecules are phosphorylated on Ser-24, half on Ser-25.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta-casein antibody
    • CASB antibody
    • CASB_HUMAN antibody
    • Casein beta antibody
    • CSN 2 antibody
    • Csn2 antibody
    see all

Images

  • ICC/IF image of ab112595 stained T24/83 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab112595 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-beta Casein antibody (ab112595) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 25 kDa

References

This product has been referenced in:
  • Blans K  et al. Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography. J Extracell Vesicles 6:1294340 (2017). WB . Read more (PubMed: 28386391) »
  • Zonneveld MI  et al. Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures. J Extracell Vesicles 3:N/A (2014). WB ; Human . Read more (PubMed: 25206958) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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