Overview

  • Product name
    Anti-beta Catenin antibody
    See all beta Catenin primary antibodies
  • Description
    Rabbit polyclonal to beta Catenin
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human beta Catenin aa 768-781 (C terminal).
    Sequence:

    PGDSNQLAWFDTDL

  • Positive control
    • Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab2365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
IHC-P Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:100-1:200 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
ICC/IF 1/100. (see Abreview).
IP Use a concentration of 5 µg/ml.

Target

  • Function
    Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Tissue specificity
    Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • Involvement in disease
    Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similarities
    Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • Post-translational
    modifications
    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

Anti-beta Catenin antibody images

  • ab2365 at 1/100 staining rat gastric epithelial cells by ICC/IF. The cells were fomaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 2 hours. A FITC conjugated goat anti-rabbit IgG was used as the secondary. Nuclei were counterstained with propidium iodide.

    The image shows the staining of gastric epithelial cells (left hand panel) and the staining of embyonic fibroblasts (negative control, right hand panel).

    See Abreview

  • ab2365 at 1/200 staining mouse skin tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours. An Alexa-Fluor ® 594 conjugated chicken anti-rabbit polyclonal was used as the secondary.

    See Abreview

  • Anti-beta Catenin antibody (ab2365) at 1/25 dilution + A431 cell lysate

    Predicted band size : 85 kDa
    Observed band size : 92 kDa (why is the actual band size different from the predicted?)
  • Beta Catenin was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to beta Catenin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2365.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 85kDa; beta Catenin.

References for Anti-beta Catenin antibody (ab2365)

This product has been referenced in:
  • Jiao S  et al. VGLL4 targets a TCF4-TEAD4 complex to coregulate Wnt and Hippo signalling in colorectal cancer. Nat Commun 8:14058 (2017). WB ; Human . Read more (PubMed: 28051067) »
  • McClelland Descalzo DL  et al. Glucose-Induced Oxidative Stress Reduces Proliferation in Embryonic Stem Cells via FOXO3A/ß-Catenin-Dependent Transcription of p21(cip1). Stem Cell Reports 7:55-68 (2016). IP ; Mouse . Read more (PubMed: 27411103) »

See all 11 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12, Muscle and Ovary)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
C2C12, Muscle and Ovary
Blocking step
Sea Block Blocking Agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. Francesco Elia Marino

Verified customer

Submitted Sep 23 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Myoblast/Myotubes)
Permeabilization
Yes - 70% Ethanol and 0.3% Triton in PBS
Specification
Myoblast/Myotubes
Blocking step
CAS Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 11 2016

Thank you for contacting us.

We have not tested this antibody for ChIP and do not have any data for this application.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or infor...

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Thank you for your inquiry and your patience.

Unfortunately, I am sorry to confirm we would not expect ab2365 and ab15180 to detect the Hydra beta catenin protein as the alignment is not expected to be high with the immunogens.

I ...

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Thank you for contacting us.

Your credit note ID is xxx.

I am sorry that these 2 antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, pleas...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Normal Skin)
Specification
Normal Skin
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Feb 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,5% Triton X-100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Feb 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (embryo)
Loading amount
20 µg
Specification
embryo
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 06 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6%
Username

Abcam user community

Verified customer

Submitted Apr 24 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (gastric epithelial cell)
Specification
gastric epithelial cell
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5%
Username

Dr. Jianyuan Chai

Verified customer

Submitted Nov 20 2006

1-10 of 13 Abreviews or Q&A

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