Synthetic peptide:
PGDSNQLAWFDTDL
conjugated to KLH, corresponding to amino acids 768-781 of Human or mouse ß Catenin. (Peptide available as ab16377)Our Abpromise guarantee covers the use of ab6302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | 1/2000. PubMed: 18624906 | |
Dot blot | 1/4000. | |
IHC-Fr | 1/2000. | |
WB | 1/4000. Predicted molecular weight: 94 kDa. | |
IHC-P | Use at an assay dependent concentration. | |
IHC - Wholemount | 1/200. |
Immunohistochemical staining of paraffin embedded, PFA fixed, mouse liver using ab6302 at 1/200. Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6, and the sample was permeabilized with 0.05% Triton X 100. The sample was blocked in 5% BSA for 1 hour at room temperature and was then incubated with primary antibody for 16 hours in PBS/0.05% Triton X 100 at 4 °C. An Alexa Fluor® 488 donkey anyti-rabbit was used as the secondary at 1/250.
IHC-Wholemount image of anti-beta Catenin antibody (ab6302) staining on the Zebrafish jaw of a 5 day old larva. This larva was stained with anti-beta Catenin antibody (green) and a collagen 2 antibody (red) for 2 hours at 20°C. The image shows the cartilage elements of the jaw.
This picture was kindly supplied as part of the review submitted by Mohaiza Dashwood. Immunofluorescence of H29 cells stained with DAPI (blue) and rabbit polyclonal anti-beta-catenin (ab6302), 1/2000) with Alexa Fluor 488 (green) from Molecular Probes.
ab6302 at 1/2000 dilution staining beta Catenin in human HeLa cells by immunocytochemistry/ immunofluorescence. Sections were paraformaldehyde fixed, permeabilized in 0.3% Triton X prior to blocking in 5% BSA for 2 hours at 27°C and then incubated with ab6302 for 8 hour at 4°C. Alexa fluor® 488 goat polyclonal, diluted 1/1000, was used as the secondary antibody. Counterstaining with DAPI.
ab6302 at 1/500 dilution staining beta Catenin in human breast cells by immunocytochemistry/ immunofluorescence. Sections were formaldehyde fixed, permeabilized in 0.5% digitonin prior to incubating with ab6302 for 1 hour. Alexa fluor® 488 goat polyclonal, diluted 1/500, was used as the secondary antibody. Treated samples recieved 10um GSK-3 Inhibitor X.
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