Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer, pH6.0
Sample
Dog Tissue sections (colon)
Specification
colon
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: PBS plus 1xcasein
Dilution
1/250

Secondary antibody

Name
Non-Abcam antibody was used: biotinylated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Username

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Submitted May 22 2013

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