Western blot abreview for Anti-beta Catenin antibody [E247]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS cell line)
Loading amount 30 µg
Specification U2OS cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution 1/5000
Incubation time 12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk 0.1% Tween-20 in PBS

Secondary antibody

Name Non-Abcam antibody was used: Swine anti-rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/5000

Detection

Detection method ECL+
Exposure 3 minute(s) and 0 second(s)
Bands Specific: ~90 kDa Non-specific: none kDa

Additional data

Additional Notes High quality antibody
Username

Abcam user community

Verified customer

Submitted Oct 09 2007