Western blot abreview for Anti-beta Catenin antibody [E247]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cell line)
Loading amount
30 µg
Specification
U2OS cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/5000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk 0.1% Tween-20 in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Swine anti-rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ~90 kDa Non-specific: none kDa

Additional data

Additional Notes
High quality antibody
Username

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Submitted Oct 09 2007

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