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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human beta Catenin aa 1-100 (N terminal).
A trial size is available to purchase for this antibody.
Alternative versions available:
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab32572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 86 kDa).|
ab32572 staining beta-catenin in the bEnd.5 murine cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton in PBS and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/300) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
ab32572 staining beta Catenin in Dog colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM citrate buffer, pH6. Samples were incubated with primary antibody (1/250 in PBS with 1x casein) for 90 minutes at 25°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Western blot image of ab32572 staining whole cell lysate of U2OS cells. The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted 1/5000 and incubated for 12 hours at 4°C. A HRP conjugated swine anti-rabbit antibody was used as the secondary.
ab32572 at 1/200 staining mouse small intestine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the primary antibody. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ab32572 staining beta Catenin in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocked with 5% serum at 40C for 30 minutes. The sample was incubated with primary antibody (1/200) in dilution buffer containing PBS and 3% Goat Serum at 40C for 9 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.
ab32572 staining human renal carcinoma tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking with 1% milk for 45 minutes at 22°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 22°C. A HRP conjugated goat anti-rabbit antibody, diluted 1/400, was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"